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<p>Endophytic fungi establish a symbiotic relationship with their host plants, actively engaging in the hosts' physiological and metabolic processes. They can directly or indirectly transform plant metabolites, thereby playing a crucial role in the host's overall health and functioning. In this study, we isolated and identified an endophytic fungus, <i>Candolleomyces candolleanus</i> P9 strain, which produces β-glucosidase from <i>Ural Glycyrrhizae Radix</i> in Altay, Xinjiang Uygur Autonomous Region, China. In addition, the enzyme production conditions of strain P9 were optimized using a wheat bran concentration of 30.6&#xa0;g/L, beef extract concentration of 11.2&#xa0;g/L, inoculum size of 2.6%, pH 7.23, at 30&#xa0;°C, with shaking at 150&#xa0;rpm, and a fermentation duration of 6&#xa0;days. Under these conditions, the β-glucosidase activity of strain P9 increased by 13.6-fold compared to the initial level. On this basis, the efficiency of converting diurea-based urea into diurea-based elements was further optimized. The optimized results were as follows: conversion time 12&#xa0;h, temperature 37℃, liquiritin concentration 0.8&#xa0;mg/mL, pH value 7.5, and the conversion rate reached 93.09%. In addition, the antibacterial and antioxidant effects of the fermentation broth of the P9 strain after biotransformation were significantly better than those of commercial β-glucosidase and control group. In summary, fermentation with the β-glucosidase-producing <i>Candolleomyces candolleanus</i> P9 strain is a potential method for converting liquiritin into liquiritigenin of <i>Glycyrrhiza uralensis Fisch</i>.</p>

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Candolleomyces candolleanus P9 from Altay Glycyrrhiza: β-glucosidase-mediated biotransformation

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年1月30日

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  • Qing Wang,
  • Yang Li,
  • Kunyi Li,
  • Xinxin Huang,
  • Marhaba Ahmat,
  • Yuanyuan Yan,
  • Xin Huang,
  • Huaibing Yao,
  • Weidong Cui,
  • Min Hou

摘要

Endophytic fungi establish a symbiotic relationship with their host plants, actively engaging in the hosts' physiological and metabolic processes. They can directly or indirectly transform plant metabolites, thereby playing a crucial role in the host's overall health and functioning. In this study, we isolated and identified an endophytic fungus, Candolleomyces candolleanus P9 strain, which produces β-glucosidase from Ural Glycyrrhizae Radix in Altay, Xinjiang Uygur Autonomous Region, China. In addition, the enzyme production conditions of strain P9 were optimized using a wheat bran concentration of 30.6 g/L, beef extract concentration of 11.2 g/L, inoculum size of 2.6%, pH 7.23, at 30 °C, with shaking at 150 rpm, and a fermentation duration of 6 days. Under these conditions, the β-glucosidase activity of strain P9 increased by 13.6-fold compared to the initial level. On this basis, the efficiency of converting diurea-based urea into diurea-based elements was further optimized. The optimized results were as follows: conversion time 12 h, temperature 37℃, liquiritin concentration 0.8 mg/mL, pH value 7.5, and the conversion rate reached 93.09%. In addition, the antibacterial and antioxidant effects of the fermentation broth of the P9 strain after biotransformation were significantly better than those of commercial β-glucosidase and control group. In summary, fermentation with the β-glucosidase-producing Candolleomyces candolleanus P9 strain is a potential method for converting liquiritin into liquiritigenin of Glycyrrhiza uralensis Fisch.