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<p>This study explores how <i>Pseudomonas putida</i> EM2-4 metabolizes linear alkanes after acquiring an integrative and conjugative element (ICE) encoding a novel alkane monooxygenase (AlkB). This AlkB enzyme is a fusion of a hydroxylase and a fatty acid desaturase, exhibiting a narrow substrate range limited to <i>n</i>-octane and <i>n</i>-decane. The oxidation of these hydrocarbons yields octanoate and decanoate, respectively, which then enter the β-oxidation pathway and the glyoxylate shunt. Under nitrogen-limiting conditions, excess carbon is redirected toward polyhydroxyalkanoate (PHA) synthesis, a phenomenon particularly pronounced in a <i>phaZ</i>-deficient mutant unable to depolymeraze PHA, leading to up 75% of cell dry weight. Analysis of the PHAs monomer composition revealed variations with the carbon source. Additionally, <sup>13</sup>C-isotopic labeling uncovered a minor but unexpected fraction of C10 monomers from C8 substrates, potentially arised from gluconeogenesis followed by the de novo synthesis of fatty acids. Proteomic analyses showed that the carbon source influences the activation of metabolic pathways, correlating with the observed variations in PHAs monomer composition.</p>

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Redirecting linear hydrocarbon metabolism toward polyhydroxyalkanoate biosynthesis

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年1月19日

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  • Rocío Palacios-Ferrer,
  • María T. Manoli,
  • Patricia Godoy,
  • Antonio Delgado,
  • Auxiliadora Prieto,
  • Juan L. Ramos

摘要

This study explores how Pseudomonas putida EM2-4 metabolizes linear alkanes after acquiring an integrative and conjugative element (ICE) encoding a novel alkane monooxygenase (AlkB). This AlkB enzyme is a fusion of a hydroxylase and a fatty acid desaturase, exhibiting a narrow substrate range limited to n-octane and n-decane. The oxidation of these hydrocarbons yields octanoate and decanoate, respectively, which then enter the β-oxidation pathway and the glyoxylate shunt. Under nitrogen-limiting conditions, excess carbon is redirected toward polyhydroxyalkanoate (PHA) synthesis, a phenomenon particularly pronounced in a phaZ-deficient mutant unable to depolymeraze PHA, leading to up 75% of cell dry weight. Analysis of the PHAs monomer composition revealed variations with the carbon source. Additionally, 13C-isotopic labeling uncovered a minor but unexpected fraction of C10 monomers from C8 substrates, potentially arised from gluconeogenesis followed by the de novo synthesis of fatty acids. Proteomic analyses showed that the carbon source influences the activation of metabolic pathways, correlating with the observed variations in PHAs monomer composition.