Scholarlink

Background <p>Interest in microbial biosurfactants has increased due to the rising demand for environmentally friendly and sustainable surfactants. Waste frying oil provides a renewable and low-cost feedstock for their production. This study aimed to isolate, characterize, and evaluate the antibacterial mechanism of a biosurfactant synthesized by <i>Achromobacter xylosoxidans</i> PX106473 using waste frying oil as an economical carbon source.</p> Results <p><i>Achromobacter xylosoxidan</i>s PX106473 produced a biosurfactant with significant activity, including an emulsification index (E24%) of 66.7% against kerosene and substantial oil displacement and hemolytic activities. According to the results of thin-layer chromatography (TLC), the produced biosurfactant contained lipids and amino acids. Fourier transform infrared spectroscopy (FT-IR) results revealed the presence of an N-H group, aliphatic hydrocarbons, and amide peaks, which suggest a lipid-peptide linkage, providing further evidence for its putative lipopeptide nature. Hexadecanoic acid, with an area percentage of 76.44, was the dominant component of the lipopeptide based on gas chromatography-mass spectrometry (GC-MS) results. The produced biosurfactant demonstrated good inhibitory activity against <i>E. coli</i> and <i>S. aureus</i>. These biological findings were further supported by in silico assays; molecular docking studies showed that hexadecanoic acid binds stably to key bacterial proteins from <i>E. coli</i> (DNA gyrase B, -6.4&#xa0;kcal/mol) and <i>S. aureus</i> (PBP2a, -3.9&#xa0;kcal/mol), indicating a potential dual-target mechanism.</p> Conclusion <p><i>Achromobacter xylosoxidans</i> efficiently produced a putative lipopeptide biosurfactant from waste frying oil with strong emulsifying and antibacterial properties, providing an economical and sustainable solution with potential in various environmental and pharmaceutical applications.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Low-cost biosurfactant production by Achromobacter xylosoxidans PX106473 from waste frying oil: partial characterization and antimicrobial mechanism via molecular docking

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年1月10日

查看全文
  • Sally M. Elmogy,
  • Magda M. Awad,
  • A. M. M. Elattaapy,
  • El Sayed F. El-Halawany,
  • Ashraf A. Elsayed

摘要

Background

Interest in microbial biosurfactants has increased due to the rising demand for environmentally friendly and sustainable surfactants. Waste frying oil provides a renewable and low-cost feedstock for their production. This study aimed to isolate, characterize, and evaluate the antibacterial mechanism of a biosurfactant synthesized by Achromobacter xylosoxidans PX106473 using waste frying oil as an economical carbon source.

Results

Achromobacter xylosoxidans PX106473 produced a biosurfactant with significant activity, including an emulsification index (E24%) of 66.7% against kerosene and substantial oil displacement and hemolytic activities. According to the results of thin-layer chromatography (TLC), the produced biosurfactant contained lipids and amino acids. Fourier transform infrared spectroscopy (FT-IR) results revealed the presence of an N-H group, aliphatic hydrocarbons, and amide peaks, which suggest a lipid-peptide linkage, providing further evidence for its putative lipopeptide nature. Hexadecanoic acid, with an area percentage of 76.44, was the dominant component of the lipopeptide based on gas chromatography-mass spectrometry (GC-MS) results. The produced biosurfactant demonstrated good inhibitory activity against E. coli and S. aureus. These biological findings were further supported by in silico assays; molecular docking studies showed that hexadecanoic acid binds stably to key bacterial proteins from E. coli (DNA gyrase B, -6.4 kcal/mol) and S. aureus (PBP2a, -3.9 kcal/mol), indicating a potential dual-target mechanism.

Conclusion

Achromobacter xylosoxidans efficiently produced a putative lipopeptide biosurfactant from waste frying oil with strong emulsifying and antibacterial properties, providing an economical and sustainable solution with potential in various environmental and pharmaceutical applications.