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Background <p>Producing bio-based chemicals using a straightforward and ecologically responsible biotechnological method is intriguing. Maltobionic acid (MBA) is an aldobionic acid obtained from maltose oxidation and is an industrially applied compound. Having antioxidative and antimicrobial, non-toxic, highly water soluble, moisturizing, metal chelating, mildly sour and slightly sweet characteristics.</p> Results <p>This study succeeded in utilizing <i>Aspergillus awamori</i> as a novel MBA producer. MBA production was improved through two-step statistical factorial designs. Plackett-Burman Design (PBD) investigating the qualitative interaction between eleven factors (maltose, KNO<sub>3</sub>, NaCl, KH<sub>2</sub>PO<sub>4</sub>, K<sub>2</sub>HPO<sub>4</sub>, MgSO<sub>4</sub>, FeSO<sub>4</sub>.7H<sub>2</sub>O, initial pH, temperature, incubation time and rpm) on MBA production causing 1.37- fold increase. Central Composite design (CCD) analyzing the quantitative interaction between the most MBA production affecting factors (temperature, rpm, KH<sub>2</sub>PO<sub>4</sub>, and incubation period) gave a 1.64- improvement in MBA production compared with un-optimized medium. The addition of agro-industrial wastes (AIW) (corn cobs and artichoke leaves) to optimized medium g/l (maltose, 5; KNO<sub>3</sub>, 1; KH<sub>2</sub>PO<sub>4</sub>, 1.0; MgSO<sub>4</sub>, 0.5; FeSO<sub>4</sub>.7(H<sub>2</sub>O), 0.02; pH, 5.00 at 37.5&#xa0;°C for 9.5 days at 125&#xa0;rpm) caused 2.41- and 1.97- fold increase in MBA production, respectively in comparison with the initial production conditions. MBA produced by <i>A. awamori</i> MH2 exerted an anti-oxidant activity with a ratio of 86% using DPPH scavenging assay.</p>

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Aspergillus awamori MH2 as a novel maltobionic acid producer: production optimization and application

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年8月11日

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  • Soad M. Yehia,
  • Yousseria M. Shetaia,
  • Ghoson M. Daba,
  • Faten A. Mostafa,
  • Ali A. Ali,
  • Hassaan El-Menoufy

摘要

Background

Producing bio-based chemicals using a straightforward and ecologically responsible biotechnological method is intriguing. Maltobionic acid (MBA) is an aldobionic acid obtained from maltose oxidation and is an industrially applied compound. Having antioxidative and antimicrobial, non-toxic, highly water soluble, moisturizing, metal chelating, mildly sour and slightly sweet characteristics.

Results

This study succeeded in utilizing Aspergillus awamori as a novel MBA producer. MBA production was improved through two-step statistical factorial designs. Plackett-Burman Design (PBD) investigating the qualitative interaction between eleven factors (maltose, KNO3, NaCl, KH2PO4, K2HPO4, MgSO4, FeSO4.7H2O, initial pH, temperature, incubation time and rpm) on MBA production causing 1.37- fold increase. Central Composite design (CCD) analyzing the quantitative interaction between the most MBA production affecting factors (temperature, rpm, KH2PO4, and incubation period) gave a 1.64- improvement in MBA production compared with un-optimized medium. The addition of agro-industrial wastes (AIW) (corn cobs and artichoke leaves) to optimized medium g/l (maltose, 5; KNO3, 1; KH2PO4, 1.0; MgSO4, 0.5; FeSO4.7(H2O), 0.02; pH, 5.00 at 37.5 °C for 9.5 days at 125 rpm) caused 2.41- and 1.97- fold increase in MBA production, respectively in comparison with the initial production conditions. MBA produced by A. awamori MH2 exerted an anti-oxidant activity with a ratio of 86% using DPPH scavenging assay.