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<p>Konjac mannan oligosaccharides (KMOS) are currently popular in food additives for their health benefits. However, the simple and efficient preparation of KMOS is still a challenge. In this study, A novel gene encoding β-mannanase (<i>CsMan134</i>) from <i>Cellvibrio sp.</i> KY-GH-1 was displayed on the surface of <i>E. coli</i> cells. Subsequently, <i>E.coli</i> cells (3&#xa0;g/L) expressing the mannanase CsMan134 were immobilized using 8% (w/v) polyvinyl alcohol, 3% (w/v) sodium alginate, and 3.5% (w/v) Fe₃O₄ to construct magnetic cross-linked cell aggregates (mag-CLCAs). The mannanase CsMan134 demonstrated the highest catalytic efficiency towards konjac mannan compared to other mannans. Compared to free enzyme, the mag-CLCAs exhibited enhanced enzymatic activity across a range of temperatures and pH levels. Furthermore, the mag-CLCAs showed improved thermal stability, retaining over 80% of its initial activity after heating at 50&#xa0;°C for 180&#xa0;min, whereas the free enzyme retained only 50% of its residual activity. Scanning electron microscopy (SEM) and vibrating sample magnetometry (VSM) analyses indicated that the mag-CLCAs also maintained good operational stability, retaining more than 75% of their initial activity over five cycles. The mag-CLCAs were effective in converting konjac mannan into a substantial amount of oligosaccharides with a degree of polymerization (DP) of 2–4. In conclusion, the mag-CLCAs represent a valuable, efficient, and cost-effective biocatalyst for the production of KMOS for industrial applications.</p>

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Harnessing magnetic cross-linked cell aggregates (CLCAs) for cost-effective preparation of Konjac mannan-oligosaccharide

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年6月24日

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  • Baoyu Cui,
  • Haiqiang Lu,
  • Xue Liu,
  • Yiran Zhang,
  • Xinxi Gu

摘要

Konjac mannan oligosaccharides (KMOS) are currently popular in food additives for their health benefits. However, the simple and efficient preparation of KMOS is still a challenge. In this study, A novel gene encoding β-mannanase (CsMan134) from Cellvibrio sp. KY-GH-1 was displayed on the surface of E. coli cells. Subsequently, E.coli cells (3 g/L) expressing the mannanase CsMan134 were immobilized using 8% (w/v) polyvinyl alcohol, 3% (w/v) sodium alginate, and 3.5% (w/v) Fe₃O₄ to construct magnetic cross-linked cell aggregates (mag-CLCAs). The mannanase CsMan134 demonstrated the highest catalytic efficiency towards konjac mannan compared to other mannans. Compared to free enzyme, the mag-CLCAs exhibited enhanced enzymatic activity across a range of temperatures and pH levels. Furthermore, the mag-CLCAs showed improved thermal stability, retaining over 80% of its initial activity after heating at 50 °C for 180 min, whereas the free enzyme retained only 50% of its residual activity. Scanning electron microscopy (SEM) and vibrating sample magnetometry (VSM) analyses indicated that the mag-CLCAs also maintained good operational stability, retaining more than 75% of their initial activity over five cycles. The mag-CLCAs were effective in converting konjac mannan into a substantial amount of oligosaccharides with a degree of polymerization (DP) of 2–4. In conclusion, the mag-CLCAs represent a valuable, efficient, and cost-effective biocatalyst for the production of KMOS for industrial applications.