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Background <p>Vacuole and mitochondria patch (vCLAMP) protein Mcp1 is crucial in eukaryotic cells response to environmental stress, but the mechanism of Mcp1 in <i>Saccharomyces boulardii</i> (<i>S. boulardii</i>) against pathogenic fungi is unclear.</p> Results <p>This work first explored the role of Mcp1 in <i>S. boulardii</i> against <i>Candida albicans</i> (<i>C. albicans</i>). The results showed that Mcp1 located on the vacuolar and mitochondrial membrane of <i>S. boulardii</i>. Overexpression of Mcp1 inhibited the adhesion and hyphal formation of <i>C. albicans</i> in vitro. The mice model of intestinal infection revealed that WT-<i>pGK1-MCP1</i> mutant enhanced the ability of <i>S. boulardii</i> antagonize <i>C. albicans</i> infecting gut. High performance liquid chromatography-mass spectrometry analysis demonstrated that overexpressing Mcp1 promoted the production of 2-phenylethanol. The latter is a secondary metabolite of <i>S. boulardii</i>, and can inhibit the adhesion and biofilm formation of <i>C. albicans</i>. The reverse transcription polymerase chain reaction and western blotting results confirmed Mcp1 promoted the production of 2-phenylethanol by regulating the expression level of Aro10. Notably, RNA-sequencing and Gene Ontology enrichment analyses showed that 2-phenylethanol impaired the oxidative stress response of <i>C. albicans</i>.</p> Conclusion <p>This work reveals the critical role of Mcp1 in <i>S. boulardii</i> against <i>C. albicans</i> by regulating 2-phenylethanol metabolism, which provide a theoretical basis for <i>S. boulardii</i> as antifungal biologic therapy to prevent and treat of <i>Candida</i> infection.</p>

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Vacuole and mitochondria patch protein Mcp1 of Saccharomyces boulardii impairs the oxidative stress response of Candida albicans by regulating 2-phenylethanol

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年4月22日

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  • Yunyun Wei,
  • Xiaohui Zhao,
  • Chuanqi Li,
  • Jianhao Fu,
  • Wanli Gao,
  • Xiaolong Mao

摘要

Background

Vacuole and mitochondria patch (vCLAMP) protein Mcp1 is crucial in eukaryotic cells response to environmental stress, but the mechanism of Mcp1 in Saccharomyces boulardii (S. boulardii) against pathogenic fungi is unclear.

Results

This work first explored the role of Mcp1 in S. boulardii against Candida albicans (C. albicans). The results showed that Mcp1 located on the vacuolar and mitochondrial membrane of S. boulardii. Overexpression of Mcp1 inhibited the adhesion and hyphal formation of C. albicans in vitro. The mice model of intestinal infection revealed that WT-pGK1-MCP1 mutant enhanced the ability of S. boulardii antagonize C. albicans infecting gut. High performance liquid chromatography-mass spectrometry analysis demonstrated that overexpressing Mcp1 promoted the production of 2-phenylethanol. The latter is a secondary metabolite of S. boulardii, and can inhibit the adhesion and biofilm formation of C. albicans. The reverse transcription polymerase chain reaction and western blotting results confirmed Mcp1 promoted the production of 2-phenylethanol by regulating the expression level of Aro10. Notably, RNA-sequencing and Gene Ontology enrichment analyses showed that 2-phenylethanol impaired the oxidative stress response of C. albicans.

Conclusion

This work reveals the critical role of Mcp1 in S. boulardii against C. albicans by regulating 2-phenylethanol metabolism, which provide a theoretical basis for S. boulardii as antifungal biologic therapy to prevent and treat of Candida infection.