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<p>Xylitol, known for its health benefits, is a valuable compound in the food and pharmaceutical industries. However, conventional chemical production methods are often unsustainable for large-scale applications, prompting the need for alternative approaches. This study demonstrates a significant enhancement in xylitol production using microbial cell factories, optimized through metabolic engineering. Two synthetic pathways were combined, and the introduction of a novel NADPH-dependent xylitol dehydrogenase further boosted xylitol yields, achieving 0.14&#xa0;g xylitol/g glucose—a record-high yield for microbial systems. Additionally, the use of sustainable feedstocks, such as glycerol and methanol, led to the production of 7000&#xa0;mg/L xylitol with a yield of 0.35&#xa0;g xylitol/g glycerol, and 250&#xa0;mg/L xylitol from methanol. These results underscore the potential for eco-friendly, cost-effective xylitol production, providing a robust foundation for future industrial-scale biotechnological applications.</p>

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Metabolic engineering for sustainable xylitol production from diverse carbon sources in Pichia pastoris

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年3月10日

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  • Xiaocong Lu,
  • Mingxin Chang,
  • Xiangyu Li,
  • Wenbing Cao,
  • Zhoukang Zhuang,
  • Qian Wu,
  • Tao Yu,
  • Aiqun Yu,
  • Hongting Tang

摘要

Xylitol, known for its health benefits, is a valuable compound in the food and pharmaceutical industries. However, conventional chemical production methods are often unsustainable for large-scale applications, prompting the need for alternative approaches. This study demonstrates a significant enhancement in xylitol production using microbial cell factories, optimized through metabolic engineering. Two synthetic pathways were combined, and the introduction of a novel NADPH-dependent xylitol dehydrogenase further boosted xylitol yields, achieving 0.14 g xylitol/g glucose—a record-high yield for microbial systems. Additionally, the use of sustainable feedstocks, such as glycerol and methanol, led to the production of 7000 mg/L xylitol with a yield of 0.35 g xylitol/g glycerol, and 250 mg/L xylitol from methanol. These results underscore the potential for eco-friendly, cost-effective xylitol production, providing a robust foundation for future industrial-scale biotechnological applications.