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Background <p>This study presents an improved technique for precision-cut lung slices to overcome the challenge of identifying pulmonary arteries in hypoxic pulmonary vasoconstriction.</p> Methods <p>The innovation employs the connexin 40 immunofluorescence staining to precisely identify pulmonary arterial endothelium.</p> Results <p>The results of the experiments demonstrated that the refined protocol successfully differentiated between pulmonary vessels, trachea or bronchus, and bronchial arteries in precision-cut lung slices. This differentiation was accomplished by administrating a cell tracer into the pulmonary circulation to aid in identifying specific structure. Furthermore, this study accurately distinguished pulmonary arteries from veins using CX40 immunostaining.</p> Conclusions <p>This study validates the accuracy and reliability of the improved precision-cut lung slices method in evaluating hypoxic pulmonary vasoconstriction particularly in pulmonary arteries.</p>

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An improved precision-cut lung slices technique for pulmonary vasoconstriction

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年3月6日

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  • Shengquan Luo,
  • Pu Liao,
  • Xinyu Song,
  • Xiuchuan Yue,
  • Xin Wu,
  • Rui Xiao,
  • Liping Zhu,
  • Qinghua Hu

摘要

Background

This study presents an improved technique for precision-cut lung slices to overcome the challenge of identifying pulmonary arteries in hypoxic pulmonary vasoconstriction.

Methods

The innovation employs the connexin 40 immunofluorescence staining to precisely identify pulmonary arterial endothelium.

Results

The results of the experiments demonstrated that the refined protocol successfully differentiated between pulmonary vessels, trachea or bronchus, and bronchial arteries in precision-cut lung slices. This differentiation was accomplished by administrating a cell tracer into the pulmonary circulation to aid in identifying specific structure. Furthermore, this study accurately distinguished pulmonary arteries from veins using CX40 immunostaining.

Conclusions

This study validates the accuracy and reliability of the improved precision-cut lung slices method in evaluating hypoxic pulmonary vasoconstriction particularly in pulmonary arteries.