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Background <p>Breast cancer (BC) is the most commonly diagnosed cancer in women. N<sup>6</sup>-methyladenosine (m<sup>6</sup>A) is the most prevalent internal modification in mammalian mRNAs and plays a crucial role in various biological processes. However, its function in Natural killer (NK) cells in BC remains unclear. NK cells are essential for cancer immunosurveillance. This study aims to assess m<sup>6</sup>A levels in transcripts involved in the NKG2D cytotoxicity signaling pathway in NK cells of BC patients compared to controls and find out its impact on mRNA levels. Additionally, it evaluates how deliberately altering m<sup>6</sup>A levels in NK cells affects mRNA and protein expression of NKG2D pathway genes and NK cell functionality.</p> Methods <p>m<sup>6</sup>A methylation in transcripts of NKG2D-pathway-related genes in BC patients and controls was determined using methylated RNA immunoprecipitation-reverse transcription-PCR (MERIP-RT-PCR). To deliberately alter m<sup>6</sup>A levels in primary cultured human NK cells, the m<sup>6</sup>A demethylases, FTO and ALKBH5, were knocked out using the CRISPR-CAS9 system, and FTO was inhibited using Meclofenamic acid (MA). The impact of m<sup>6</sup>A alteration on corresponding mRNA and protein levels was assessed using RT-qPCR and Western blot analysis or flow cytometry, respectively. Additionally, NK cell functionality was evaluated through degranulation and <sup>51</sup>Cr release cytotoxicity assays.</p> Results <p>Transcripts of NKG2D, an activating receptor that detects stressed non-self tumour cells, had significantly higher m<sup>6</sup>A levels in the 3′ untranslated region (3’UTR) accompanied by a marked reduction in their corresponding mRNA levels in BC patients compared to controls. Conversely, transcripts of ERK2 and PRF1 exhibited significantly lower m<sup>6</sup>A levels escorted with higher mRNA expression in BC patients relative to controls. The mRNA levels of PI3K, PAK1 and GZMH were also significantly elevated in BC patients. Furthermore, artificially increasing transcripts’ m<sup>6</sup>A levels via MA in cultured primary NK cells reduced mRNA levels of NKG2D pathway genes and death receptor ligands but did not affect protein expression or NK cell functionality.</p> Conclusion <p>Transcripts with higher m<sup>6</sup>A levels in the 3’UTR region were less abundant, and vice versa. However, changes in mRNA levels of the target genes didn’t impact their corresponding protein levels or NK cell functionality.</p>

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N6-methyladenosine RNA base modification regulates NKG2D-dependent and cytotoxic genes expression in natural killer cells

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年5月19日

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  • Raghda A. Elsabbagh,
  • Ghada Abdelhady,
  • Doris Urlaub,
  • Mina Sandusky,
  • Ola Khorshid,
  • Mohamed Z. Gad,
  • Khaled Abou-Aisha,
  • Carsten Watzl,
  • Mona Rady

摘要

Background

Breast cancer (BC) is the most commonly diagnosed cancer in women. N6-methyladenosine (m6A) is the most prevalent internal modification in mammalian mRNAs and plays a crucial role in various biological processes. However, its function in Natural killer (NK) cells in BC remains unclear. NK cells are essential for cancer immunosurveillance. This study aims to assess m6A levels in transcripts involved in the NKG2D cytotoxicity signaling pathway in NK cells of BC patients compared to controls and find out its impact on mRNA levels. Additionally, it evaluates how deliberately altering m6A levels in NK cells affects mRNA and protein expression of NKG2D pathway genes and NK cell functionality.

Methods

m6A methylation in transcripts of NKG2D-pathway-related genes in BC patients and controls was determined using methylated RNA immunoprecipitation-reverse transcription-PCR (MERIP-RT-PCR). To deliberately alter m6A levels in primary cultured human NK cells, the m6A demethylases, FTO and ALKBH5, were knocked out using the CRISPR-CAS9 system, and FTO was inhibited using Meclofenamic acid (MA). The impact of m6A alteration on corresponding mRNA and protein levels was assessed using RT-qPCR and Western blot analysis or flow cytometry, respectively. Additionally, NK cell functionality was evaluated through degranulation and 51Cr release cytotoxicity assays.

Results

Transcripts of NKG2D, an activating receptor that detects stressed non-self tumour cells, had significantly higher m6A levels in the 3′ untranslated region (3’UTR) accompanied by a marked reduction in their corresponding mRNA levels in BC patients compared to controls. Conversely, transcripts of ERK2 and PRF1 exhibited significantly lower m6A levels escorted with higher mRNA expression in BC patients relative to controls. The mRNA levels of PI3K, PAK1 and GZMH were also significantly elevated in BC patients. Furthermore, artificially increasing transcripts’ m6A levels via MA in cultured primary NK cells reduced mRNA levels of NKG2D pathway genes and death receptor ligands but did not affect protein expression or NK cell functionality.

Conclusion

Transcripts with higher m6A levels in the 3’UTR region were less abundant, and vice versa. However, changes in mRNA levels of the target genes didn’t impact their corresponding protein levels or NK cell functionality.