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Background <p><i>Devriesea agamarum</i> is a recognized pathogen of some reptile species. However, our knowledge on epidemiology as well as phenotypic and genotypic properties of this microorganism is still not fully elucidated. Therefore, the aim of the present work was to assess the occurrence of <i>D</i>. <i>agamarum</i> in clinically healthy and diseased lizards from several captive pet collections in Poland. The obtained isolates were characterised using selected biochemical and molecular methods. Owing to the clinical significance of the bacterium, the additional goal was to assess its antimicrobial susceptibility by the broth microdilution method.</p> Methods <p>Swabs from 80 lizards were subject to bacterial culture. The recovered isolates of <i>D</i>. <i>agamarum</i> were examined phenotypically (RapID™ CB PLUS commercial system and additional tests) and genotypically (ERIC-PCR and partial sequence analysis of the <i>rpo</i>B and <i>dna</i>J genes).</p> Results <p>In total, 29 isolates were obtained from 18 out of 38 sampled lizards displaying signs of infection, and from 11 out of 42 healthy individuals. The bearded dragon (<i>Pogona vitticeps</i>) and spiny-tailed lizards (members of the genus <i>Uromastyx</i>) were recognised as asymptomatic carriers (with rates of 42.1 and 33.3%, respectively). Clinical infections in the former species were detected more frequently than reported previously. The present study also confirms the potential pathogenicity of <i>D. agamarum</i> to members of the family Chamaeleonidae. The phylogenetic analysis revealed that <i>D. agamarum</i> is a heterogeneous species; based on the <i>rpo</i>B or <i>dna</i>J genes, the isolates could be divided into 2 or 3 distinct clades, respectively. The MIC50/MIC90 values (µg/ml) for ampicillin, ceftiofur, enrofloxacin, florfenicol, gentamicin, tetracyclines and trimethoprim/sulfamethoxazole were 0.5/1, 0.25/0.5, 1/&gt;1, ≤ 1/2, 8/&gt;8, 2/4 and &gt; 2/38/&gt;2/38, respectively.</p>

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Occurrence, antimicrobial susceptibility and genetic diversity of Devriesea agamarum strains isolated from captive lizard collections

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年8月4日

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  • Jarosław Król,
  • Magdalena Florek,
  • Gabriela Motyka,
  • Anna Bogucka,
  • Anna Wanecka,
  • Maja Marynowska,
  • Aleksandra Pliszczak-Król,
  • Aleksandra Napierała,
  • Sylwia Banaszkiewicz,
  • Filip Pater-Jajdelski,
  • Łukasz Skomorucha

摘要

Background

Devriesea agamarum is a recognized pathogen of some reptile species. However, our knowledge on epidemiology as well as phenotypic and genotypic properties of this microorganism is still not fully elucidated. Therefore, the aim of the present work was to assess the occurrence of D. agamarum in clinically healthy and diseased lizards from several captive pet collections in Poland. The obtained isolates were characterised using selected biochemical and molecular methods. Owing to the clinical significance of the bacterium, the additional goal was to assess its antimicrobial susceptibility by the broth microdilution method.

Methods

Swabs from 80 lizards were subject to bacterial culture. The recovered isolates of D. agamarum were examined phenotypically (RapID™ CB PLUS commercial system and additional tests) and genotypically (ERIC-PCR and partial sequence analysis of the rpoB and dnaJ genes).

Results

In total, 29 isolates were obtained from 18 out of 38 sampled lizards displaying signs of infection, and from 11 out of 42 healthy individuals. The bearded dragon (Pogona vitticeps) and spiny-tailed lizards (members of the genus Uromastyx) were recognised as asymptomatic carriers (with rates of 42.1 and 33.3%, respectively). Clinical infections in the former species were detected more frequently than reported previously. The present study also confirms the potential pathogenicity of D. agamarum to members of the family Chamaeleonidae. The phylogenetic analysis revealed that D. agamarum is a heterogeneous species; based on the rpoB or dnaJ genes, the isolates could be divided into 2 or 3 distinct clades, respectively. The MIC50/MIC90 values (µg/ml) for ampicillin, ceftiofur, enrofloxacin, florfenicol, gentamicin, tetracyclines and trimethoprim/sulfamethoxazole were 0.5/1, 0.25/0.5, 1/>1, ≤ 1/2, 8/>8, 2/4 and > 2/38/>2/38, respectively.