Scholarlink

Background <p>Chicken infectious anemia virus (CIAV) causes immunosuppression, aplastic anemia, and lymphoid atrophy in young chicks, causing major economic losses to the poultry industry. Vaccination is the primary strategy for disease control. This study aimed to develop a ferritin-based nanoparticle vaccine displaying CIAV VP1-VP2 proteins and evaluate its protective efficacy in chicks.</p> Results <p>Using the SpyTag003/SpyCatcher003 system, we expressed ferritin-SpyCatcher003 (SC-FN) in insect cells and SpyTag003-VP2-VP1Nd129 in <i>E. coli</i>, achieving in vitro assembly of FN-VP2-VP1Nd129 nanoparticles, which were confirmed by SDS-PAGE, Western blotting, and transmission electron microscopy. Two-week-old chicks immunized with the nanoparticle vaccine developed significantly higher CIAV-specific antibody levels compared to the control group. Following lethal challenge with CIAV Cux-1 strain, the vaccine group showed significantly higher hematocrit values and thymus indices (<i>p</i> &lt; 0.001), and importantly, no viral nucleic acid was detected in their thymus tissues.</p> Conclusions <p>We successfully constructed a ferritin nanoparticle-based antigen display system for CIAV. The vaccine induced robust humoral immunity, effectively protected against viral replication and clinical disease, and offers a promising new strategy for developing safe and effective subunit vaccines against CIAV.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Development and immunological evaluation of a ferritin nanoparticle vaccine displaying chicken infectious anemia virus VP1-VP2 proteins

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年7月2日

查看全文
  • Lulu Zhang,
  • Zhijun Li,
  • Yu-Mei Chen Yan,
  • Hailiang Sun,
  • Fanhua Wei

摘要

Background

Chicken infectious anemia virus (CIAV) causes immunosuppression, aplastic anemia, and lymphoid atrophy in young chicks, causing major economic losses to the poultry industry. Vaccination is the primary strategy for disease control. This study aimed to develop a ferritin-based nanoparticle vaccine displaying CIAV VP1-VP2 proteins and evaluate its protective efficacy in chicks.

Results

Using the SpyTag003/SpyCatcher003 system, we expressed ferritin-SpyCatcher003 (SC-FN) in insect cells and SpyTag003-VP2-VP1Nd129 in E. coli, achieving in vitro assembly of FN-VP2-VP1Nd129 nanoparticles, which were confirmed by SDS-PAGE, Western blotting, and transmission electron microscopy. Two-week-old chicks immunized with the nanoparticle vaccine developed significantly higher CIAV-specific antibody levels compared to the control group. Following lethal challenge with CIAV Cux-1 strain, the vaccine group showed significantly higher hematocrit values and thymus indices (p < 0.001), and importantly, no viral nucleic acid was detected in their thymus tissues.

Conclusions

We successfully constructed a ferritin nanoparticle-based antigen display system for CIAV. The vaccine induced robust humoral immunity, effectively protected against viral replication and clinical disease, and offers a promising new strategy for developing safe and effective subunit vaccines against CIAV.