Scholarlink

Background <p>There is increasing evidence that gut microbiota is associated with cardiovascular health. In this exploratory study, the 16&#xa0;S rRNA amplicon sequencing was employed to investigate the composition and differential abundances of bacterial gut microbiota of dogs at different stages of myxomatous mitral valve disease (MMVD): dogs with congestive heart failure (CHF, <i>n</i> = 38), dogs in the preclinical stage of the disease (non-CHF, <i>n</i> = 23) and healthy controls with no apparent heart disease (<i>n</i> = 17). Flow cytometry was performed to quantify T lymphocytes and their subtypes, as well as monocytes, natural killer (NK) cells and B lymphocytes. Concentrations of selected chemokines, N-terminal pro-B-type natriuretic peptide (NT-proBNP) and C-reactive protein (CRP) were also measured. Correlation analysis was performed between immunological parameters and bacterial taxa of the gut microbiota.</p> Results <p>Alpha diversity did not differ significantly between the study groups; however, several differentially abundant taxa were identified. <i>Escherichia</i>/<i>Shigella</i> was overabundant in the CHF group and showed a positive correlation with activated T lymphocyte levels, whereas <i>Megamonas</i> was overabundant in the control group and was negatively correlated with monocytes and NT-proBNP levels. <i>Lachnospiraceae</i> was overabundant in the non-CHF group.</p> Conclusions <p>These findings suggest that dogs with varying severity of heart disease differ in gut microbiota composition. The observed associations between microbiota profiles, immunological parameters and disease status indicate potential microbiome–immune interactions in disease progression.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Exploratory analysis of gut microbiota and its association with peripheral blood immune cell populations in canine myxomatous mitral valve disease

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年6月16日

查看全文
  • Martina Cimerman,
  • Bojan Papić,
  • Alenka Nemec Svete,
  • Jana Avberšek,
  • Darja Kušar,
  • Sara Petek,
  • Miha Bajc,
  • Katka Pohar,
  • Alojz Ihan,
  • Aleksandra Domanjko Petrič

摘要

Background

There is increasing evidence that gut microbiota is associated with cardiovascular health. In this exploratory study, the 16 S rRNA amplicon sequencing was employed to investigate the composition and differential abundances of bacterial gut microbiota of dogs at different stages of myxomatous mitral valve disease (MMVD): dogs with congestive heart failure (CHF, n = 38), dogs in the preclinical stage of the disease (non-CHF, n = 23) and healthy controls with no apparent heart disease (n = 17). Flow cytometry was performed to quantify T lymphocytes and their subtypes, as well as monocytes, natural killer (NK) cells and B lymphocytes. Concentrations of selected chemokines, N-terminal pro-B-type natriuretic peptide (NT-proBNP) and C-reactive protein (CRP) were also measured. Correlation analysis was performed between immunological parameters and bacterial taxa of the gut microbiota.

Results

Alpha diversity did not differ significantly between the study groups; however, several differentially abundant taxa were identified. Escherichia/Shigella was overabundant in the CHF group and showed a positive correlation with activated T lymphocyte levels, whereas Megamonas was overabundant in the control group and was negatively correlated with monocytes and NT-proBNP levels. Lachnospiraceae was overabundant in the non-CHF group.

Conclusions

These findings suggest that dogs with varying severity of heart disease differ in gut microbiota composition. The observed associations between microbiota profiles, immunological parameters and disease status indicate potential microbiome–immune interactions in disease progression.