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Background <p>Cryopreservation of buck spermatozoa is an efficient strategy to improve qualified sperm cells for artificial insemination; however, this process potentially decreases the qualitative characteristics of post-thawed sperm. Therefore, this research aimed to evaluate the influence of the mitochondria-targeted antioxidant “Mito-TEMPO” on buck sperm quality during the cryopreservation process in a plant-based extender.</p> Results <p>This study indicated that the dosages 10 and 100 µM Mito-TEMPO presented higher (<i>P</i> ≤ 0.05) percentages of total motility, progressive motility, membrane integrity, mitochondrial activity, acrosome integrity, and viability while showing lower (<i>P</i> ≤ 0.05) late apoptosis, DNA fragmentation, reactive oxygen species (ROS) concentration, and lipid peroxidation compared to the control group. Different treatments of Mito-TEMPO indicated no effect (<i>P</i> &gt; 0.05) on sperm cells with abnormal morphology.</p> Conclusions <p>It can be concluded that this mitochondria-targeted antioxidant may be able to preserve the qualitative parameters in the post-thawed buck spermatozoa.</p>

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Qualitative and flow cytometric evaluations of goat’s frozen semen in Mito-TEMPO supplemented plant-based extender

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年4月30日

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  • Fatemeh Zarei,
  • Nader Asadzadeh,
  • Hassan Khamisabadi,
  • Hoda Javaheri Barfourooshi,
  • Ali Hatefi,
  • Reza Masoudi,
  • Mohsen Sharafi

摘要

Background

Cryopreservation of buck spermatozoa is an efficient strategy to improve qualified sperm cells for artificial insemination; however, this process potentially decreases the qualitative characteristics of post-thawed sperm. Therefore, this research aimed to evaluate the influence of the mitochondria-targeted antioxidant “Mito-TEMPO” on buck sperm quality during the cryopreservation process in a plant-based extender.

Results

This study indicated that the dosages 10 and 100 µM Mito-TEMPO presented higher (P ≤ 0.05) percentages of total motility, progressive motility, membrane integrity, mitochondrial activity, acrosome integrity, and viability while showing lower (P ≤ 0.05) late apoptosis, DNA fragmentation, reactive oxygen species (ROS) concentration, and lipid peroxidation compared to the control group. Different treatments of Mito-TEMPO indicated no effect (P > 0.05) on sperm cells with abnormal morphology.

Conclusions

It can be concluded that this mitochondria-targeted antioxidant may be able to preserve the qualitative parameters in the post-thawed buck spermatozoa.