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<p><i>Staphylococcus aureus</i> (<i>S. aureus</i>) is one of major pathogens in animal production. <i>S. aureus</i>-induced mastitis poses a serious threat to animal health and leads to significant economic losses. The objective of this study was to isolate and characterize bacteriophage(s) specific to <i>S. aureus</i> and assess its (their) in vitro antibacterial activity. A strain of <i>S. aureus</i> bacteriophage, designated JXAU-SA-P1, was isolated from sewage in a commercial pig farm. Morphological analysis revealed that JXAU-SA-P1 belongs to the <i>Caudovimetes</i> class. The genome of JXAU-SA-P1 is 134.23&#xa0;kb in length with a GC content of 29.99%, and no virulence or antibiotic resistance genes were identified. JXAU-SA-P1 exhibited a low optimal multiplicity of infection (0.001), a high burst size (300 PFU/cell), and significant antibacterial activity. The bacteriophage showed stable activity below 50&#xa0;°C and within a pH range of 2.0–12.0. JXAU-SA-P1 effectively inhibited the growth of <i>S. aureus</i> in milk at both 4&#xa0;°C and 25&#xa0;°C. Furthermore, it demonstrated substantial biofilm prevention and removal properties. In conclusion, these findings indicate that JXAU-SA-P1 is a potential antibacterial agent for the control of <i>S. aureus</i>-induced mastitis.</p>

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Isolation and characterization of a lytic bacteriophage against Staphylococcus aureus

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年2月28日

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  • Yi Liu,
  • Minfeng Ding,
  • Tiande Zou,
  • Xingping Chen,
  • Xin Li,
  • Yan Chen,
  • Jun Chen,
  • Jinming You

摘要

Staphylococcus aureus (S. aureus) is one of major pathogens in animal production. S. aureus-induced mastitis poses a serious threat to animal health and leads to significant economic losses. The objective of this study was to isolate and characterize bacteriophage(s) specific to S. aureus and assess its (their) in vitro antibacterial activity. A strain of S. aureus bacteriophage, designated JXAU-SA-P1, was isolated from sewage in a commercial pig farm. Morphological analysis revealed that JXAU-SA-P1 belongs to the Caudovimetes class. The genome of JXAU-SA-P1 is 134.23 kb in length with a GC content of 29.99%, and no virulence or antibiotic resistance genes were identified. JXAU-SA-P1 exhibited a low optimal multiplicity of infection (0.001), a high burst size (300 PFU/cell), and significant antibacterial activity. The bacteriophage showed stable activity below 50 °C and within a pH range of 2.0–12.0. JXAU-SA-P1 effectively inhibited the growth of S. aureus in milk at both 4 °C and 25 °C. Furthermore, it demonstrated substantial biofilm prevention and removal properties. In conclusion, these findings indicate that JXAU-SA-P1 is a potential antibacterial agent for the control of S. aureus-induced mastitis.