Scholarlink

Background <p>Porcine deltacoronavirus (PDCoV) is a newly emerging coronavirus that causes severe diarrhea and dehydration in newborn piglets, and can infect multiple animal species including humans, posing a substantial threat to global swine production and human health. To address these challenges, we developed a droplet digital PCR (ddPCR) method for detecting the <i>N</i> gene of PDCoV in clinical samples.</p> Results <p>Our ddPCR method exhibited good linearity, reproducibility, and repeatability. In comparison to conventional quantitative real-time PCR (qPCR), the ddPCR assay demonstrated exceptional sensitivity, with limits of detection (LoD) of 1.65 copies/reaction (95% CI: 1.39–2.14) for clinical sample and 1.62 copies/reaction (95% CI: 1.36–2.12) for standard plasmid, respectively. Besides, the ddPCR assay displayed no cross-reactivity with the other 6 common swine pathogens. The results of clinical sample testing showed that the positivity rate of ddPCR (29/182) was greater than that of qPCR (25/182), with a coincidence rate of 97.80%.</p> Conclusions <p>The established ddPCR method exhibits good sensitivity, repeatability, and specificity, indicating that this method can be used as a promising tool for the sensitive detection and quantification of PDCoV.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Establishment and application of a droplet digital PCR assay for sensitive detection of porcine deltacoronavirus

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年12月5日

查看全文
  • Xueying Han,
  • Yangkun Liu,
  • Yan Wang,
  • Jie Zhang,
  • Guangxue Ding,
  • Yutao Li,
  • Siming Xu,
  • Jiashen Zhang,
  • Lunguang Yao

摘要

Background

Porcine deltacoronavirus (PDCoV) is a newly emerging coronavirus that causes severe diarrhea and dehydration in newborn piglets, and can infect multiple animal species including humans, posing a substantial threat to global swine production and human health. To address these challenges, we developed a droplet digital PCR (ddPCR) method for detecting the N gene of PDCoV in clinical samples.

Results

Our ddPCR method exhibited good linearity, reproducibility, and repeatability. In comparison to conventional quantitative real-time PCR (qPCR), the ddPCR assay demonstrated exceptional sensitivity, with limits of detection (LoD) of 1.65 copies/reaction (95% CI: 1.39–2.14) for clinical sample and 1.62 copies/reaction (95% CI: 1.36–2.12) for standard plasmid, respectively. Besides, the ddPCR assay displayed no cross-reactivity with the other 6 common swine pathogens. The results of clinical sample testing showed that the positivity rate of ddPCR (29/182) was greater than that of qPCR (25/182), with a coincidence rate of 97.80%.

Conclusions

The established ddPCR method exhibits good sensitivity, repeatability, and specificity, indicating that this method can be used as a promising tool for the sensitive detection and quantification of PDCoV.