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Background <p><i>Lactobacillus salivarius</i> (<i>L. salivarius</i>) is a probiotic widely colonized in the gastrointestinal tracts of humans and animals. Its biosurfactant (BS) exhibits antibacterial and antiviral activities, along with advantages such as biodegradability, non-toxicity, and stability. Porcine circovirus type 2 (PCV2) is a major pathogen in swine farms, causing immunosuppression or immune dysfunction. PCV2-positive herds exhibit elevated susceptibility, often accompanied by severe secondary bacterial infections. To explore novel antimicrobial biologics for enhancing swine immunity, this study isolated a biosurfactant-producing <i>Lactobacillus</i> strain from the intestines of PCV2-infected piglets using the calcium carbonate plate method, and analyzed the anti-microbial activity of the biosurfactant.</p> Results <p>A strain of biosurfactant-producing <i>L. salivarius</i> was successfully isolated from the ileum of PCV2-infected piglets. The extracted BS from the isolate demonstrated surface tension-reducing properties, forming spreading zones or dispersions on vegetable oil. The antimicrobial experiment showed that at the concentrations of 1.56 − 50&#xa0;mg/mL, BS inhibited the growth of porcine <i>E. coli</i> and <i>S. suis</i> by 46.01% − 95.06% and 41.06% − 81.64%, respectively. Furthermore, BS of 6.25–12.5&#xa0;mg/mL significantly inhibited PCV2 replication in PK-15 cells. These findings suggest that <i>L. salivarius</i> is a promising probiotic candidate for improving swine immune status and developing novel alternatives to conventional antimicrobial agents.</p>

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Isolation of porcine intestinal Lactobacillus salivarius and antimicrobial potential of its biosurfactant

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年8月26日

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  • Xiaotian Zhou,
  • Jing Ren,
  • Chen Yuan,
  • Feiyan Wang,
  • Ligong Chen,
  • Xiaobo Wang,
  • Liu Yang,
  • Lizhi Fu,
  • Qinye Song

摘要

Background

Lactobacillus salivarius (L. salivarius) is a probiotic widely colonized in the gastrointestinal tracts of humans and animals. Its biosurfactant (BS) exhibits antibacterial and antiviral activities, along with advantages such as biodegradability, non-toxicity, and stability. Porcine circovirus type 2 (PCV2) is a major pathogen in swine farms, causing immunosuppression or immune dysfunction. PCV2-positive herds exhibit elevated susceptibility, often accompanied by severe secondary bacterial infections. To explore novel antimicrobial biologics for enhancing swine immunity, this study isolated a biosurfactant-producing Lactobacillus strain from the intestines of PCV2-infected piglets using the calcium carbonate plate method, and analyzed the anti-microbial activity of the biosurfactant.

Results

A strain of biosurfactant-producing L. salivarius was successfully isolated from the ileum of PCV2-infected piglets. The extracted BS from the isolate demonstrated surface tension-reducing properties, forming spreading zones or dispersions on vegetable oil. The antimicrobial experiment showed that at the concentrations of 1.56 − 50 mg/mL, BS inhibited the growth of porcine E. coli and S. suis by 46.01% − 95.06% and 41.06% − 81.64%, respectively. Furthermore, BS of 6.25–12.5 mg/mL significantly inhibited PCV2 replication in PK-15 cells. These findings suggest that L. salivarius is a promising probiotic candidate for improving swine immune status and developing novel alternatives to conventional antimicrobial agents.