Scholarlink

Background <p>African swine fever (ASF) is a highly lethal infectious disease caused by the African swine fever virus (ASFV). Due to the absence of effective vaccines and antiviral drugs, the slaughter of infected and exposed pigs is a current prevention and control measure. Early diagnosis is crucial for the management of ASFV, and the p15 protein plays a critical role in the maturation of ASFV particles.</p> Results <p>In this study, a prokaryotic expression system obtained highly pure soluble p15 protein, and four monoclonal antibodies (3&#xa0;C, 4B, 5D, 8&#xa0;F) were prepared. These antibodies demonstrated the specific recognition of exogenously expressed p15. Antigenic epitopes were initially mapped using six overlapping p15 truncated proteins. Results showed that the epitopes of 3&#xa0;C, 5D, and 8&#xa0;F were all located in P49-N65, which is highly conserved among genotypes I, II, VII, IX, X, and XX. In contrast, 4B identified an epitope located at K105-R132, which was conserved only in genotypes I, II, and XX ASFV.</p> Conclusions <p>This study identified two novel conserved epitopes, P49-N65 and K105-R132. This provided valuable insights into the antigenic epitopes of ASFV p15 protein, contributing to a better understanding of its functional properties and demonstrating potential utility for the development of ASFV diagnostic tools.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Preparation and identification of a novel monoclonal antibody against African swine fever virus p15 protein

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年8月6日

查看全文
  • Jian He,
  • Mingzhan Luo,
  • Mengyang Zhang,
  • Xiaomin Hu,
  • Yangkun Liu,
  • Lunguang Yao

摘要

Background

African swine fever (ASF) is a highly lethal infectious disease caused by the African swine fever virus (ASFV). Due to the absence of effective vaccines and antiviral drugs, the slaughter of infected and exposed pigs is a current prevention and control measure. Early diagnosis is crucial for the management of ASFV, and the p15 protein plays a critical role in the maturation of ASFV particles.

Results

In this study, a prokaryotic expression system obtained highly pure soluble p15 protein, and four monoclonal antibodies (3 C, 4B, 5D, 8 F) were prepared. These antibodies demonstrated the specific recognition of exogenously expressed p15. Antigenic epitopes were initially mapped using six overlapping p15 truncated proteins. Results showed that the epitopes of 3 C, 5D, and 8 F were all located in P49-N65, which is highly conserved among genotypes I, II, VII, IX, X, and XX. In contrast, 4B identified an epitope located at K105-R132, which was conserved only in genotypes I, II, and XX ASFV.

Conclusions

This study identified two novel conserved epitopes, P49-N65 and K105-R132. This provided valuable insights into the antigenic epitopes of ASFV p15 protein, contributing to a better understanding of its functional properties and demonstrating potential utility for the development of ASFV diagnostic tools.