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Introduction and purpose <p>Brucellosis is a prevalent bacterial zoonosis globally, affecting a broad range of hosts. The role of rodents in the survival and transmission of <i>Brucella</i> species to humans remains uncertain. This study aimed to investigate the prevalence of <i>Brucella</i> infection among wild rodents in western Iran, specifically in KabudarAhang County within Hamadan Province.</p> Materials and methods <p>Sampling was conducted across various regions of KabudarAhang County in western Iran between April 2014 and September 2017. Serological testing was performed using the standard tube agglutination method while molecular investigation was carried out through real-time PCR analysis. Subsequently, molecularly positive samples underwent species identification via conventional PCR.</p> Results <p>Serological testing revealed 7 positive samples (1.76%), including four <i>Meriones persicus</i>, one <i>Mus musculus</i>, one <i>Meriones libycus</i>, and one <i>Spermophilus fulvus</i>. In the molecular survey, three samples (0.68%) tested positive for <i>Brucella</i>; these included one <i>M. musculus</i> and two <i>M. persicus</i>. The molecular species identification test detected <i>B. abortus</i> in <i>M. musculus</i> among those positive for <i>Brucella</i>.</p> Conclusion <p>These findings suggest that wild rodents may play an overlooked role in the maintenance of pathogenic <i>Brucella</i> species in natural environments. Furthermore, in situations where standard diagnostic guidelines for brucellosis in wildlife are not well established, employing multiple testing approaches is crucial for accurate detection.</p>

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Molecular and serological survey of Brucella spp. among rodents in western Iran

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年4月3日

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  • Majid Hemati,
  • Mohammad Khalili,
  • Saber Esmaeili,
  • Ahmad Mahmoudi,
  • Hossein Ahangari Cohan,
  • Ehsan Mostafavi

摘要

Introduction and purpose

Brucellosis is a prevalent bacterial zoonosis globally, affecting a broad range of hosts. The role of rodents in the survival and transmission of Brucella species to humans remains uncertain. This study aimed to investigate the prevalence of Brucella infection among wild rodents in western Iran, specifically in KabudarAhang County within Hamadan Province.

Materials and methods

Sampling was conducted across various regions of KabudarAhang County in western Iran between April 2014 and September 2017. Serological testing was performed using the standard tube agglutination method while molecular investigation was carried out through real-time PCR analysis. Subsequently, molecularly positive samples underwent species identification via conventional PCR.

Results

Serological testing revealed 7 positive samples (1.76%), including four Meriones persicus, one Mus musculus, one Meriones libycus, and one Spermophilus fulvus. In the molecular survey, three samples (0.68%) tested positive for Brucella; these included one M. musculus and two M. persicus. The molecular species identification test detected B. abortus in M. musculus among those positive for Brucella.

Conclusion

These findings suggest that wild rodents may play an overlooked role in the maintenance of pathogenic Brucella species in natural environments. Furthermore, in situations where standard diagnostic guidelines for brucellosis in wildlife are not well established, employing multiple testing approaches is crucial for accurate detection.