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Background <p>Bovine diarrhea virus (BVDV) is considered to be the most common pathogen causing severe diarrhea in cattle worldwide and can cause Bovine viral diarrhea (BVD). Clinical manifestations of fever, diarrhea, ulcers, and abortions, resulting in significant economic losses to the cattle industry. The development of an efficient, rapid and sensitive assay suitable for field conditions is of great significance for its early detection. Recombinase polymerase amplification (RPA) is a novel nucleic acid amplification method that has been widely used in the diagnosis of infectious diseases.</p> Results <p>We developed a rapid assay (RPAS) combining RPA with SYBR Green I for the detection of BVDV. The BVDV RPAS assay was performed at 37&#xa0;°C in 25&#xa0;min. The minimum detection limit of the RPAS assay is 1 × 10<sup>9</sup> copies/µL in sunlight and 1 × 10<sup>5</sup> copies/µL in ultraviolet light, and there is no cross-reactivity with other viruses that cause gastrointestinal and respiratory infections in cattle. The coincidence rate of BVDV RPAS in clinical samples was higher than that of PCR.</p> Conclusions <p>The BVDV RPAS assay established in this study has high sensitivity and specificity, and is expected to be a powerful tool for the prevention and control of BVD.</p>

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Rapid visual detection of bovine viral diarrhea virus (BVDV) using recombinase polymerase amplification with SYBR green I

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年4月2日

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  • Lingling Jiang,
  • Pu Wang,
  • Gang Zhang,
  • Xiaoxia Niu,
  • Qiang Liu,
  • Ruijin Liang,
  • Sinong Zhang,
  • Yong Li

摘要

Background

Bovine diarrhea virus (BVDV) is considered to be the most common pathogen causing severe diarrhea in cattle worldwide and can cause Bovine viral diarrhea (BVD). Clinical manifestations of fever, diarrhea, ulcers, and abortions, resulting in significant economic losses to the cattle industry. The development of an efficient, rapid and sensitive assay suitable for field conditions is of great significance for its early detection. Recombinase polymerase amplification (RPA) is a novel nucleic acid amplification method that has been widely used in the diagnosis of infectious diseases.

Results

We developed a rapid assay (RPAS) combining RPA with SYBR Green I for the detection of BVDV. The BVDV RPAS assay was performed at 37 °C in 25 min. The minimum detection limit of the RPAS assay is 1 × 109 copies/µL in sunlight and 1 × 105 copies/µL in ultraviolet light, and there is no cross-reactivity with other viruses that cause gastrointestinal and respiratory infections in cattle. The coincidence rate of BVDV RPAS in clinical samples was higher than that of PCR.

Conclusions

The BVDV RPAS assay established in this study has high sensitivity and specificity, and is expected to be a powerful tool for the prevention and control of BVD.