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Background <p><i>Glaesserella parasuis</i> (<i>G. parasuis</i>) is one of the most important porcine pathogens causing Glässer’s disease, and QseBC two-component system (TCS) is associated with various behaviors of <i>G. parasuis</i>. Our preliminary tests confirmed that plant-derived compounds cinnamaldehyde (CAL) and hesperitin (HES) exhibited promising antimicrobial activity against <i>G. parasuis</i>. Here, we further investigate the antimicrobial effects of CAL and HES on <i>G. parasuis</i> and the underlying mechanisms.</p> Results <p>We observed that CAL and HES affected the morphology and physiology of <i>G. parasuis</i> based on the biofilm biomass, confocal laser scanning microscope (CLSM) assay, scanning electron microscope (SEM) assay, and conductivity determination as well as intracellular iron level measurement either used alone or in combination. Moreover, CAL and HES can inhibit QseBC TCS of <i>G</i>. <i>parasuis</i> by down-regulating the QseBC related genes and quenching the QseC protein based on quantitative reverse transcription polymerase chain reaction (qRT-PCR) and molecular docking and fluorescence quenching assay. In vivo study further evident that CAL and HES exhibited significant antimicrobial and anti-inflammatory activity on <i>G. parasuis</i>-infected mice.</p> Conclusions <p>These findings suggested that CAL and HES can exert antibacterial activity on resistant <i>G. parasuis</i> by targeting QseBC, and they may serve as the promising antimicrobial agents for the treatment of <i>G. parasuis</i> infection.</p>

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Antibacterial effects of cinnamaldehyde and hesperitin on resistant Glaesserella parasuis by suppressing QseBC two-component system

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年4月12日

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  • Jingru Zuo,
  • Lin Liao,
  • Yujun Gao,
  • Junlin Chen,
  • Jiang Teng,
  • Weihua Zhang,
  • Yuhang Wang,
  • Yu Sun,
  • Xiaoqiang Liu

摘要

Background

Glaesserella parasuis (G. parasuis) is one of the most important porcine pathogens causing Glässer’s disease, and QseBC two-component system (TCS) is associated with various behaviors of G. parasuis. Our preliminary tests confirmed that plant-derived compounds cinnamaldehyde (CAL) and hesperitin (HES) exhibited promising antimicrobial activity against G. parasuis. Here, we further investigate the antimicrobial effects of CAL and HES on G. parasuis and the underlying mechanisms.

Results

We observed that CAL and HES affected the morphology and physiology of G. parasuis based on the biofilm biomass, confocal laser scanning microscope (CLSM) assay, scanning electron microscope (SEM) assay, and conductivity determination as well as intracellular iron level measurement either used alone or in combination. Moreover, CAL and HES can inhibit QseBC TCS of G. parasuis by down-regulating the QseBC related genes and quenching the QseC protein based on quantitative reverse transcription polymerase chain reaction (qRT-PCR) and molecular docking and fluorescence quenching assay. In vivo study further evident that CAL and HES exhibited significant antimicrobial and anti-inflammatory activity on G. parasuis-infected mice.

Conclusions

These findings suggested that CAL and HES can exert antibacterial activity on resistant G. parasuis by targeting QseBC, and they may serve as the promising antimicrobial agents for the treatment of G. parasuis infection.