Scholarlink

Background <p>Multidrug resistance in Escherichia coli has a significant global impact on poultry production. This study aimed to determine the phenotypic and genotypic backgrounds of antimicrobial resistance (AMR) and virulence gene profiles of <i>E. coli</i> strains isolated from diseased and healthy broilers. A total of 211 <i>E. coli</i> isolates were recovered from diseased (<i>n</i> = 110) and healthy broilers (<i>n</i> = 101). All the isolates were subjected to antimicrobial susceptibility testing. A PCR-based technique was applied to screen AMR genes, virulence genes and analyze phylogenetic groups.</p> Results <p>Phylogenetic groups B1 and D were the most prevalent for <i>E. coli</i> isolated from diseased and healthy birds. Among virulence genes, the detection rates of <i>cva/cvi</i>,<i> iutA</i>,<i> iucD</i>,<i> iroN</i>,<i> iss</i> and <i>ompT</i> were considerably greater in <i>E.coli</i> strains from diseased birds than in healthy birds. The virulence gene pattern of <i>hlyF-iutA-iucD-iroN-iss-ompT</i> (16.4%) was frequently observed in <i>E.coli</i> isolated from diseased birds, whereas approximately 22.8% of <i>E.coli</i> from healthy birds did not carry any virulence genes. Analysis of AMR profiles revealed that 58.3% of <i>E.coli</i> were resistant to multiple classes of antibiotics, and 96.7% carried at least one antibiotic resistance gene AMR genes.</p> Conclusion <p>The findings of this study demonstrate the variable distribution of phylogenetic groups and virulence genes. <i>E.coli</i> strains isolated from broilers had multidrug resistance profiles. The study emphasizes the need for continuous monitoring of AMR emergence in <i>E. coli</i> from broilers. This monitoring allows for early detection and implementation of strategies to control the spread of resistant strains.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Antimicrobial resistance, virulence gene profiles, and phylogenetic groups of Escherichia coli isolated from healthy broilers and broilers with colibacillosis in Thailand

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年3月8日

查看全文
  • Sudtisa Laopiem,
  • Kriangkrai Witoonsatian,
  • Sittinee Kulprasetsri,
  • Pun Panomwan,
  • Chutima Pathomchai-umporn,
  • Raktipon Kamtae,
  • Pichai Jirawattanapong,
  • Thaweesak Songserm,
  • Nuananong Sinwat

摘要

Background

Multidrug resistance in Escherichia coli has a significant global impact on poultry production. This study aimed to determine the phenotypic and genotypic backgrounds of antimicrobial resistance (AMR) and virulence gene profiles of E. coli strains isolated from diseased and healthy broilers. A total of 211 E. coli isolates were recovered from diseased (n = 110) and healthy broilers (n = 101). All the isolates were subjected to antimicrobial susceptibility testing. A PCR-based technique was applied to screen AMR genes, virulence genes and analyze phylogenetic groups.

Results

Phylogenetic groups B1 and D were the most prevalent for E. coli isolated from diseased and healthy birds. Among virulence genes, the detection rates of cva/cvi, iutA, iucD, iroN, iss and ompT were considerably greater in E.coli strains from diseased birds than in healthy birds. The virulence gene pattern of hlyF-iutA-iucD-iroN-iss-ompT (16.4%) was frequently observed in E.coli isolated from diseased birds, whereas approximately 22.8% of E.coli from healthy birds did not carry any virulence genes. Analysis of AMR profiles revealed that 58.3% of E.coli were resistant to multiple classes of antibiotics, and 96.7% carried at least one antibiotic resistance gene AMR genes.

Conclusion

The findings of this study demonstrate the variable distribution of phylogenetic groups and virulence genes. E.coli strains isolated from broilers had multidrug resistance profiles. The study emphasizes the need for continuous monitoring of AMR emergence in E. coli from broilers. This monitoring allows for early detection and implementation of strategies to control the spread of resistant strains.