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<p>This study aims to explore the coding sequence (CDS) of the putative <i>DUS</i> gene in <i>Eimeria media</i> and assess its potential biological functions during the parasite's lifecycle. Initially, oocysts were isolated from fecal samples of rabbits infected with <i>E. media</i>, from which DNA and RNA were extracted. These extractions were used as templates for PCR to successfully amplify the CDS of the <i>DUS</i> gene, confirming its presence within the <i>E. media</i> genome. Further analysis using quantitative PCR (qPCR) demonstrated significantly higher expression of the <i>DUS</i> gene in the precocious line (PL) compared to the wild type (WT). This differential expression highlights a potential functional role for the <i>DUS</i> gene in influencing the development and sporulation processes of <i>E. media</i>, which may contribute to the precocious phenotype. Additionally, bioinformatics analysis provided insights into the evolutionary trends and structural characteristics of the <i>DUS</i> gene across different species, suggesting a broader biological significance. The elevated expression of the <i>DUS</i> gene in the PL suggests its critical involvement in the growth and reproductive processes of the parasite. This finding opens new avenues for research aimed at controlling <i>E. media</i>&#xa0;infection through targeted interventions in the <i>DUS</i> gene expression pathways.</p>

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Identification and bioinformatics analysis of the DUS gene in Eimeria media

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年1月6日

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  • Yijin Zou,
  • Yiyan Wang,
  • Diyi Duan,
  • Xun Suo,
  • Yuanyuan Zhang

摘要

This study aims to explore the coding sequence (CDS) of the putative DUS gene in Eimeria media and assess its potential biological functions during the parasite's lifecycle. Initially, oocysts were isolated from fecal samples of rabbits infected with E. media, from which DNA and RNA were extracted. These extractions were used as templates for PCR to successfully amplify the CDS of the DUS gene, confirming its presence within the E. media genome. Further analysis using quantitative PCR (qPCR) demonstrated significantly higher expression of the DUS gene in the precocious line (PL) compared to the wild type (WT). This differential expression highlights a potential functional role for the DUS gene in influencing the development and sporulation processes of E. media, which may contribute to the precocious phenotype. Additionally, bioinformatics analysis provided insights into the evolutionary trends and structural characteristics of the DUS gene across different species, suggesting a broader biological significance. The elevated expression of the DUS gene in the PL suggests its critical involvement in the growth and reproductive processes of the parasite. This finding opens new avenues for research aimed at controlling E. media infection through targeted interventions in the DUS gene expression pathways.