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Background <p><i>Pseudomonas</i> <i>fluorescens</i> is a Gram-negative bacterium with a remarkable metabolic and physiological versatility that enables it to adapt and colonize diverse ecological niches, including the human small intestine. While serotonin is primarily found in high concentrations in gut tissue, its levels in the lumen can be elevated in conditions such as celiac disease, where <i>P. fluorescens</i> is also found in increased abundance. The potential effects of serotonin on <i>P. fluorescens</i> in such contexts remain unclear.</p> Results <p>We demonstrate that <i>P. fluorescens</i> metabolizes serotonin primarily into 5-hydroxyindole-3-acetic acid (5-HIAA) and, to a lesser extent, into 5-hydroxytryptophol and N-acetylserotonin. Gene expression analysis revealed significant changes in oxidative stress-related pathways over time, and proteomic analysis confirmed the shifts seen particularly in amino acid catabolic pathways. Serotonin metabolism also enhanced bacterial resistance to oxidative stress, suggesting a protective role.</p> Conclusions <p>The findings reveal a novel mechanism by which serotonin modulates the metabolism and stress responses of <i>P. fluorescens</i>. This study provides insight into how <i>P. fluorescens</i> adapts to serotonin-rich environments, such as in celiac disease, and may inform future research on microbial interactions with host-derived metabolites in disease contexts.</p>

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Serotonin modulation of metabolism and stress response in Pseudomonas fluorescens

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年10月31日

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  • Barbora Waclawiková,
  • Markus Schwalbe,
  • Diana Ilyaskina,
  • Semih Toptas,
  • Nicola U. Thome,
  • Chao Du,
  • Somayah S. Elsayed,
  • Anne de Jong,
  • Gilles P. van Wezel,
  • Sahar El Aidy

摘要

Background

Pseudomonas fluorescens is a Gram-negative bacterium with a remarkable metabolic and physiological versatility that enables it to adapt and colonize diverse ecological niches, including the human small intestine. While serotonin is primarily found in high concentrations in gut tissue, its levels in the lumen can be elevated in conditions such as celiac disease, where P. fluorescens is also found in increased abundance. The potential effects of serotonin on P. fluorescens in such contexts remain unclear.

Results

We demonstrate that P. fluorescens metabolizes serotonin primarily into 5-hydroxyindole-3-acetic acid (5-HIAA) and, to a lesser extent, into 5-hydroxytryptophol and N-acetylserotonin. Gene expression analysis revealed significant changes in oxidative stress-related pathways over time, and proteomic analysis confirmed the shifts seen particularly in amino acid catabolic pathways. Serotonin metabolism also enhanced bacterial resistance to oxidative stress, suggesting a protective role.

Conclusions

The findings reveal a novel mechanism by which serotonin modulates the metabolism and stress responses of P. fluorescens. This study provides insight into how P. fluorescens adapts to serotonin-rich environments, such as in celiac disease, and may inform future research on microbial interactions with host-derived metabolites in disease contexts.