Background <p>Physical activity plays an important role in maintaining the health and performance of nurses, especially those working in outpatient room (OR) and emergency room (ER) that has a high workload. However, data on the level of physical activity and its influencing factors among these nurses is unclear.</p> Purpose <p>This study aimed to determine and compare the physical activity levels of ER and OR nurses and the relationship between physical activity levels and work-related demographic factors (age, length of work, unit room/department, gender, and career level).</p> Patients and methods <p>Our study used a correlational design with 117 nurses (57 ER and 60 OR nurses). This study used the Global Physical Activity Questionnaire (GPAQ) as the instrument. Data were analyzed with the Spearman test.</p> Results <p>A total of 117 nurses completed the survey. Most participants reported high physical activity level (<i>n</i> = 77, 65.8%), low (<i>n</i> = 24, 20.5%) and moderate (<i>n</i> = 16, 13,7%). Furthermore, the study found a significant weak positive correlation between physical activity with age (<i>r</i> = 0.25, <i>p</i> = 0.006) and length of work (<i>r</i> = 0.24, <i>p</i> = 0.010). But no significant correlation were found with gender, career level and unit room (<i>p</i> &gt; 0.05).</p> Conclusion <p>The findings suggest that age and length of work are significantly associated with physical activity levels among emergency and outpatient nurses. These results highlight the need for age- and experience-specific strategies to encourage and maintain physical activity in the nursing workforce.</p>