Objectives <p>To assess the effect of video-based educational program on staff nurses’ awareness and practices regarding risk factors and prevention of exposure keratopathy (EK) among mechanically ventilated and unconscious patients.</p> Methods <p>This study employed a quasi-experimental, pretest-posttest interventional design involving a sample of 109 intensive care unit (ICU) nurses currently employed in private hospitals.</p> Results <p>Univariate analyses revealed a significant relationship between participants’ age, gender, and years of experience with their practice at the post-test. Additionally, there was a significant improvement in participants’ awareness after the intervention for the total mean score and all items (<i>p</i> &lt; 0.001), except for question 8: “Are patients with incomplete lid closure referred to an ophthalmologist?” (<i>p</i> = 0.063). Regarding ICU nurses’ practices in eye care, there was a significant improvement in all items after the intervention (<i>p</i> &lt; 0.001).</p> Conclusion <p>The findings of this study revealed that the educational intervention significantly improved ICU nurses’ awareness and practices regarding the risk factors and prevention of EK among mechanically ventilated and unconscious patients. Moreover, these findings emphasize the effectiveness of educational interventions in improving nurses’ awareness and their ability to effectively implement preventive measures.</p> Clinical trial number <p>Not applicable.</p>