Background <p>In recent years, nurses’ turnover has become a major problem, especially in underdeveloped and developing countries. This research aims to reveal the mediating role of job satisfaction in the effect of nurse-nurse collaboration on turnover intention.</p> Methods <p>This study is a quantitative, descriptive, cross-sectional study. This study was conducted in three private hospitals within a healthcare group in Turkey. The research was conducted with the participation of 534 nurses. These data were analyzed using moderated mediation regressions with the SPSS PROCESS macro.</p> Results <p>Nurse-nurse collaboration had a significant effect on turnover intention and job satisfaction. Job satisfaction had a significant effect on turnover intention. The mediating effect of job satisfaction on the effect of nurse-nurse collaboration on turnover intention was significant.</p> Conclusions <p>Our findings show that nurse-nurse collaboration and job satisfaction are important for reducing turnover intention. It shows that job satisfaction is an important mediating variable in the effect of nurse-nurse collaboration on reducing turnover intention.</p> Clinical trial number <p>Not applicable</p>