Background <p>Scenario-based animations are e-learning tools that offer significant contributions to students to transform their learning into action by influencing their perceptions and feelings. The study aimed to evaluate the effects of a scenario-based range of motion exercise animation on nursing students' knowledge, motivation, and attitudes.</p> Methods <p>The randomized controlled experimental study was conducted in December 2021. The students were randomly assigned to experimental (n=52) and control (n=52) groups. Data were obtained with the “Student Information Form”, “Range of Motion Exercises Knowledge Test”,“E-Learning Motivation Scale”, and “E-Learning Attitude Scale”. The study was carried out in two stages. First, the scenario-based animation video was created. In the second, the experimental group was trained with the scenario-based animation video, and the control group with the basic skill video. Ethics committee approval was obtained (Date:21/12/2020, Number: 24237859-775).</p> Results <p>Both groups’ knowledge levels increased in the post-test (p = 0.001), but there was no statistically significant difference in the knowledge levels between the groups (p &gt; 0.001). Besides, the motivation and attitude scores of the students in the experimental group were statistically significantly higher than those of the students in the control group in the post-test (<i>p</i> &lt; 0.05).</p> Conclusion <p>Scenario-based animation videos in nursing education are instructive and have a positive effect on developing high motivation and attitude in students. Scenario-based animation videos should be integrated into the nursing education curriculum as course material.</p>