<p>The present study aimed to determine the antioxidant, antimicrobial, cytotoxic and anticarcinogenic activities, wound-healing properties, element levels and phytochemical profiles of aqueous extracts of <i>Verbascum splendidum</i> leaf and aboveground parts (VSE_leaf and VSE_mix). Total phenolic content, total flavonoid content, total antioxidant capacity, total oxidant capacity levels and oxidative stress index were analyzed as the antioxidant parameters. The total phenolic contents of VSE_mix and VSE_leaf were comparable. While VSE_mix exhibited higher total flavonoid levels, this difference did not reach statistical significance. Oxidative stress index of VSE_mix extract was lower than VSE_leaf extract. The high amount and variety of phytochemical content, high total phenolic and total flavonoid amounts in VSE_mix extract support this situation. Moreover, these extracts, especially VSE_mix, were rich in bio-elements such as Fe, Cu, Mn and Zn which have roles on antioxidant system. <i>E. coli, S. aureus</i> and <i>C. albicans</i> strains were used to detect antimicrobial activity. The VSE_mix extract, which was rich in phenolic content, had higher antimicrobial activity than VSE_leaf extract. The cytotoxic effects of plant extracts were determined using L929 cell line, the anticarcinogenic effects of plant extracts were determined using A549. VSE_mix had anticarcinogenic activity for A549 cells and VSE_leaf for G361 cells, and both extracts had a positive effect on wound-healing compared to the control group. The findings demonstrated that especially VSE_mix could serve as a valuable natural source of bioactive compounds for prospective clinical use due to its antioxidant, antimicrobial, anticancerogenic, cytotoxic effects or wound-healing activity.</p>