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Aim <p>In this study, we investigated the potential of hydroxyapatite nanoparticles (HA-NP) synthesized from <i>Elaeagnus Angustifolia</i> (HAEA-NP) and loaded with crocin (HAEA-NP@CR) to enhance the in vitro wound healing of human dermal fibroblasts (hDF).</p> Method <p>HAEA-NPs were synthesized using the sol-gel technique and impregnated with crocin (CR) to create the HAEA-NP@CR formulation. The examination of CR loading and release was performed. The assessment of viability of hDFs following exposure to nanoparticles (NPs), CR, and HAEA-NP@CR was assessed. A scratch assay was performed to assess the rate of wound closure. Real-time PCR analysis was utilized to measure the expression levels of genes associated with wound healing.</p> Result <p>According to our findings, CR had a loading capacity of 46.76%. It was released in a controlled manner from HAEA-NPs. The combination of HAEA-NP and CR significantly promoted the viability of hDFs and accelerated wound closure in an in vitro wound healing model. Furthermore, we observed an upregulation of key wound healing-related genes underlying the enhanced wound healing effect.</p> Conclusion <p>These findings suggest that the use of HAEA-NP@CR holds promise as a novel approach to promote wound healing and may have potential applications in developing wound healing therapies.</p>

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Enhanced in vitro wound healing of human dermal fibroblasts using nanohydroxyapatite synthesized from Elaeagnus Angustifolia and loaded with crocin

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年10月22日

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  • Ehsaneh Azaryan,
  • Akram Ghodousi,
  • Mohammad Yahya Hanafi-Bojd ,
  • Mohsen Naseri

摘要

Aim

In this study, we investigated the potential of hydroxyapatite nanoparticles (HA-NP) synthesized from Elaeagnus Angustifolia (HAEA-NP) and loaded with crocin (HAEA-NP@CR) to enhance the in vitro wound healing of human dermal fibroblasts (hDF).

Method

HAEA-NPs were synthesized using the sol-gel technique and impregnated with crocin (CR) to create the HAEA-NP@CR formulation. The examination of CR loading and release was performed. The assessment of viability of hDFs following exposure to nanoparticles (NPs), CR, and HAEA-NP@CR was assessed. A scratch assay was performed to assess the rate of wound closure. Real-time PCR analysis was utilized to measure the expression levels of genes associated with wound healing.

Result

According to our findings, CR had a loading capacity of 46.76%. It was released in a controlled manner from HAEA-NPs. The combination of HAEA-NP and CR significantly promoted the viability of hDFs and accelerated wound closure in an in vitro wound healing model. Furthermore, we observed an upregulation of key wound healing-related genes underlying the enhanced wound healing effect.

Conclusion

These findings suggest that the use of HAEA-NP@CR holds promise as a novel approach to promote wound healing and may have potential applications in developing wound healing therapies.