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Objectives <p>This study evaluated the coronal dentinal tubule penetration of three calcium silicate-based materials used in regenerative endodontic procedures.</p> Materials and methods <p>Sixty extracted human mandibular premolars were sectioned 5&#xa0;mm from the apex and prepared with Peeso reamers to simulate immature apices. Regenerative procedures followed AAE guidelines. Before placement of calcium hydroxide, canals were irrigated with 20 mL 2.5% NaOCl for 5&#xa0;min and 20 mL 17% EDTA for 5&#xa0;min, respectively. The canals were sealed with Cavit and incubated for four weeks. In the second visit simulation, the temporary restoration was removed. After removal and irrigation with 17% EDTA, human blood was introduced 3–4&#xa0;mm below the cemento-enamel junction, and a collagen matrix was placed over the clot. Samples were randomly divided into three groups (<i>n</i> = 20) and capped with Biodentine, Dia-Root Bio MTA, or Bio-C Repair, each mixed with 0.01% rhodamine B. After incubation at 37&#xa0;°C and 100% humidity for two weeks, 2-mm coronal root sections were analyzed with the Cytation™ 5 Cell Imaging Multimode Reader. Dentinal tubule penetration was measured at four standardized points using ImageJ. Statistical analysis used the Shapiro-Wilk, ANOVA, and Duncan tests (<i>p</i> &lt; 0.05).</p> Results <p>Biodentine and Bio-C Repair showed significantly greater maximum penetration depths than Dia-Root Bio MTA (<i>p</i> = 0.001). Bio-C Repair also exhibited the highest mean penetration depth, significantly higher than the other materials (<i>p</i> = 0.026).</p> Conclusions <p>Bio-C Repair demonstrated superior coronal dentinal tubule penetration compared with Biodentine and Dia-Root Bio MTA.</p> Clinical relevance <p>Bio-C Repair may be a reliable material for regenerative endodontic procedures requiring an effective coronal seal.</p>

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Coronal tubule penetration of different calcium silicate materials in regenerative endodontics: a comparative study with a novel imaging method

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年1月3日

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  • Ayse Karadayi,
  • Betul Aycan Uysal,
  • Burcin Arican Alpay

摘要

Objectives

This study evaluated the coronal dentinal tubule penetration of three calcium silicate-based materials used in regenerative endodontic procedures.

Materials and methods

Sixty extracted human mandibular premolars were sectioned 5 mm from the apex and prepared with Peeso reamers to simulate immature apices. Regenerative procedures followed AAE guidelines. Before placement of calcium hydroxide, canals were irrigated with 20 mL 2.5% NaOCl for 5 min and 20 mL 17% EDTA for 5 min, respectively. The canals were sealed with Cavit and incubated for four weeks. In the second visit simulation, the temporary restoration was removed. After removal and irrigation with 17% EDTA, human blood was introduced 3–4 mm below the cemento-enamel junction, and a collagen matrix was placed over the clot. Samples were randomly divided into three groups (n = 20) and capped with Biodentine, Dia-Root Bio MTA, or Bio-C Repair, each mixed with 0.01% rhodamine B. After incubation at 37 °C and 100% humidity for two weeks, 2-mm coronal root sections were analyzed with the Cytation™ 5 Cell Imaging Multimode Reader. Dentinal tubule penetration was measured at four standardized points using ImageJ. Statistical analysis used the Shapiro-Wilk, ANOVA, and Duncan tests (p < 0.05).

Results

Biodentine and Bio-C Repair showed significantly greater maximum penetration depths than Dia-Root Bio MTA (p = 0.001). Bio-C Repair also exhibited the highest mean penetration depth, significantly higher than the other materials (p = 0.026).

Conclusions

Bio-C Repair demonstrated superior coronal dentinal tubule penetration compared with Biodentine and Dia-Root Bio MTA.

Clinical relevance

Bio-C Repair may be a reliable material for regenerative endodontic procedures requiring an effective coronal seal.