Introduction <p>Environmental degradation from pollution, global warming, and industrialization, is alarming with dentistry significantly contributing through mercury and plastic toxicity from improper waste handling.</p> Aim <p>To evaluate the knowledge, attitude, and practice of Dental professionals towards “Green Dentistry” working in the public and private sectors of Karachi city.</p> Design <p>A cross-sectional survey.</p> Setting <p>Karachi, Pakistan (Online).</p> Materials and methods <p>An online questionnaire was conducted among dentists of Karachi, Pakistan, from January to June 2023 collecting participant’s demographic data and assessing knowledge, attitude, and practice of eco-friendly dentistry.</p> Results <p>With a 61% response rate, most respondents (48.5%) were General Dental Practitioners (GDPs). Dental consultants (DC) exhibited higher awareness of green dentistry (29%) with 50% of DCs favoring digital radiography as environmentally friendly (<i>p</i> &lt; 0.05). Green dentistry practices were more prevalent among DCs with 42.8% (<i>p</i> &lt; 0.05) eliminating amalgam usage, while more GDPs (72.2%, <i>p</i> &lt; 0.05) adopted re-usable lab coats and patient drapes.</p> Discussion and conclusion <p>Awareness of “Green Dentistry” is generally lacking among participants. Both GDPs and DCs are inclined towards adopting environment-friendly dental practices. Education on energy conservation and materials is essential to reduce waste in the dental industry.</p>