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Aim <p>This study aimed to assess the influence of Bioactive glass nanoparticle (BAG-np) and Chitosan nanoparticle (CSNP) as intracanal medications compared with triple antibiotic paste (TAP) on the release of TGF-β1 from radicular dentin via enzyme-linked immunosorbent assay (ELISA).</p> Methods <p>Forty dentin discs (1-mm thick) were horizontally cut from roots of 40 teeth.The samples were submerged in 20&#xa0;ml of 1.5% NaOCL, followed by submersion in 20&#xa0;ml 17% of EDTA, after which they were distributed equally into 2 experimental groups (I, II) and 2 control groups (III, IV) (10 samples each). Group I: (BAG-np), group II: (CSNP), group III: (TAP) (positive control) and group IV: nonmedicated (negative control). After 3 weeks, the medications were removed via Phosphate buffer saline (PBS), and the samples were subsequently submerged in PBS for 24&#xa0;h at 37&#xa0;°C. The medium from the samples was collected and used to measure the TGF-β1 concentration via ELISA.</p> Results <p>There was a statistically significant difference between the BAG-np, CSNP, and nonmedicated groups compared to the TAP group (P-value &lt; 0.05). The highest value of released (TGF-β1) was recorded in the CSNP group, followed by the BAG-np group, then the nonmedicated group, whereas the lowest value was recorded in the TAP group.</p> Conclusion <p>BAG-np and CSNP induce the release of TGF-β1after irrigation with 1.5% NaOCl and 17%EDTA, significantly greater than TAP. Accordingly, BAG-np and CSNP may be used as alternatives to TAP in regenerative endodontic procedures. </p>

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Effect of Chitosan and bioactive glass nanomaterials as intracanal medicaments on TGF-β1 release from intraradicular dentin

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年10月16日

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  • Sarah Salah Hashem,
  • Mohammed M. Khalefa,
  • Mahmoud Hassan Mohamed,
  • Hemat M. ELSheikh,
  • Fatma Abd El-Rahman Taher

摘要

Aim

This study aimed to assess the influence of Bioactive glass nanoparticle (BAG-np) and Chitosan nanoparticle (CSNP) as intracanal medications compared with triple antibiotic paste (TAP) on the release of TGF-β1 from radicular dentin via enzyme-linked immunosorbent assay (ELISA).

Methods

Forty dentin discs (1-mm thick) were horizontally cut from roots of 40 teeth.The samples were submerged in 20 ml of 1.5% NaOCL, followed by submersion in 20 ml 17% of EDTA, after which they were distributed equally into 2 experimental groups (I, II) and 2 control groups (III, IV) (10 samples each). Group I: (BAG-np), group II: (CSNP), group III: (TAP) (positive control) and group IV: nonmedicated (negative control). After 3 weeks, the medications were removed via Phosphate buffer saline (PBS), and the samples were subsequently submerged in PBS for 24 h at 37 °C. The medium from the samples was collected and used to measure the TGF-β1 concentration via ELISA.

Results

There was a statistically significant difference between the BAG-np, CSNP, and nonmedicated groups compared to the TAP group (P-value < 0.05). The highest value of released (TGF-β1) was recorded in the CSNP group, followed by the BAG-np group, then the nonmedicated group, whereas the lowest value was recorded in the TAP group.

Conclusion

BAG-np and CSNP induce the release of TGF-β1after irrigation with 1.5% NaOCl and 17%EDTA, significantly greater than TAP. Accordingly, BAG-np and CSNP may be used as alternatives to TAP in regenerative endodontic procedures.