Background <p>Effective removal of intracanal drugs such as calcium hydroxide is important for the success of endodontic treatment and to prevent possible complications. This study aimed to evaluate the effect of modified salt solution (RISA), a new irrigation solution, in removing calcium hydroxide from artificial grooves in straight root canals.</p> Methods <p>The root canals of 60 human maxillary central incisors were prepared using Reciproc R40 files (VDW) up to size 40/0.04, rinsed with 2 mL of 2.5% NaOCl after each pecking motion, and then the teeth were split longitudinally. A lateral groove in the apical part was prepared in each root half and filled with calcium hydroxide, the root halves were reassembled and samples were placed in Eppendorf tubes. Three groups were established according to the irrigation solutions: group 1, Etilendiamintetraacetic acid (EDTA); group 2, RISA; group 3, Citric acid. After the root canals were irrigated with the irrigation solutions in each group, irrigation activation was performed with the Ultra X ultrasonic device (1&#xa0;min). The samples were removed from the Eppendorf tubes, divided into two halves, and examined under a 24× magnification microscope by the two evaluators. Statistical analysis was performed using IBM SPSS version 23. Fisher Freeman Halton Test was used to analyze categorical data (<i>p</i> &lt; 0.05).</p> Results <p>There is no statistically significant difference between the score values ​​of the groups (<i>p</i> = 0.632).</p> Conclusions <p>RISA solution was similarly effective in removing calcium hydroxide compared to EDTA and citric acid solutions in the apical region.</p>