Background <p>The current study aimed to assess the impact of periodontitis on oxidative stress parameters by examining serum total antioxidant capacity (TAS), total oxidant status (TOS), oxidative stress index (OSI), thiol/ disulfide homeostasis and ischemia modified albumin (IMA).</p> Methods <p>The study had 90 participants, categorized into 3 groups: Group 1: Periodontally healthy; Group 2: Stage II Grade B periodontitis; Group 3: Stage III and IV Grade B periodontitis. Demographic and periodontal variables were assessed. The levels of serum TAS, TOS, OSI, IMA, and thiol/disulfide were assessed.</p> Results <p>No significant differences in sex and age were detected among the groups (<i>p</i> &gt; 0.05). When compared to Group 1, all clinical measurements were statistically significantly greater in Group 3 (<i>p</i> &lt; 0.05). Statistical analysis revealed no significant differences in serum TAS, TOS, and OSI levels among the groups (<i>p</i> &gt; 0.05). The highest serum IMA value was observed in Group 3 (<i>p</i> = 0.037), whereas native thiol (<i>p</i> = 0.00), total thiol (<i>p</i> = 0.00) and disulfide values (<i>p</i> = 0.023) were highest in Group 1.</p> Conclusions <p>These findings indicate that thiol/disulfide homeostasis and IMA could hold promise as a potential biomarker of inflammation in periodontitis.</p>