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Objective <p>In this study, mitochondrial-targeted nanoparticles containing CORM-401 were designed by using TPP and HA (named as CORM-TPP@HA). The effects of these nanoparticles on cuproptosis in A549 cells were observed to confirm their function.</p> Methods <p>First, CORM-TPP micelles were formed by using CORM-401 and TPP-SH. Then, the micelles were encapsulated with HA to form nanoparticles (CORM-TPP@HA). The morphology, size distribution, stability, and release of CO from nanoparticles were analyzed to confirm their characterization. Meanwhile, the cellular uptake of nanoparticles was observed using a confocal microscope. The effects of the nanoparticles on cell viability, mitochondrial ROS, intracellular copper, and cuproptosis-related proteins in A549 cells were measured. Additionally, the impacts of nanoparticles on tumor growth and cuproptosis-related factors in tumor tissue were measured in vivo.</p> Results <p>The designed nanoparticles maintained stable formation, effectively preventing premature CO release from CORM-401. Furthermore, the nanoparticles were able to be taken up by A549 cells. Moreover, treatment with the designed nanoparticles induced cuproptosis by increasing mitochondrial ROS, intracellular copper, and cuproptosis-related proteins in A549 cells. In line with the in vitro results, treatment with nanoparticles suppressed tumor growth and elevated the cuproptosis-related proteins in tumor tissues.</p> Conclusion <p>The synthesized CORM-TPP@HA nanoparticles suppressed A549 cells via regulating mitochondrial cuproptosis, providing a signaling mechanism of CO in NSCLC treatment.</p>

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Mitochondria-targeted CORM-401 nanoparticles inhibit A549 cells by triggering cuproptosis

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年3月31日

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  • Li Shao,
  • Zhicheng Song,
  • Xiaoli Gong,
  • Jianjun Ma,
  • Shujing Ge

摘要

Objective

In this study, mitochondrial-targeted nanoparticles containing CORM-401 were designed by using TPP and HA (named as CORM-TPP@HA). The effects of these nanoparticles on cuproptosis in A549 cells were observed to confirm their function.

Methods

First, CORM-TPP micelles were formed by using CORM-401 and TPP-SH. Then, the micelles were encapsulated with HA to form nanoparticles (CORM-TPP@HA). The morphology, size distribution, stability, and release of CO from nanoparticles were analyzed to confirm their characterization. Meanwhile, the cellular uptake of nanoparticles was observed using a confocal microscope. The effects of the nanoparticles on cell viability, mitochondrial ROS, intracellular copper, and cuproptosis-related proteins in A549 cells were measured. Additionally, the impacts of nanoparticles on tumor growth and cuproptosis-related factors in tumor tissue were measured in vivo.

Results

The designed nanoparticles maintained stable formation, effectively preventing premature CO release from CORM-401. Furthermore, the nanoparticles were able to be taken up by A549 cells. Moreover, treatment with the designed nanoparticles induced cuproptosis by increasing mitochondrial ROS, intracellular copper, and cuproptosis-related proteins in A549 cells. In line with the in vitro results, treatment with nanoparticles suppressed tumor growth and elevated the cuproptosis-related proteins in tumor tissues.

Conclusion

The synthesized CORM-TPP@HA nanoparticles suppressed A549 cells via regulating mitochondrial cuproptosis, providing a signaling mechanism of CO in NSCLC treatment.