ScholarLink (思领科)
错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Unveiling potential: urinary exosomal mRNAs as non-invasive biomarkers for early prostate cancer diagnosis

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2024年8月1日

查看全文
  • Jiayin Yu,
  • Chifei Yu,
  • Kangxian Jiang,
  • Guanglin Yang,
  • Shubo Yang,
  • Shuting Tan,
  • Tingting Li,
  • Haiqi Liang,
  • Qihuan He,
  • Faye Wei,
  • Yujian Li,
  • Jiwen Cheng,
  • Fubo Wang

摘要

Background

This study investigated the use of urinary exosomal mRNA as a potential biomarker for the early detection of prostate cancer (PCa).

Methods

Next-generation sequencing was utilized to analyze exosomal RNA from 10 individuals with confirmed PCa and 10 individuals without cancer. Subsequent validation through qRT-PCR in a larger sample of 43 PCa patients and 92 healthy controls revealed distinct mRNA signatures associated with PCa.

Results

Notably, mRNAs for RAB5B, WWP1, HIST2H2BF, ZFY, MARK2, PASK, RBM10, and NRSN2 showed promise as diagnostic markers, with AUC values between 0.799 and 0.906 and significance p values. Combining RAB5B and WWP1 in an exoRNA diagnostic model outperformed traditional PSA tests, achieving an AUC of 0.923, 81.4% sensitivity, and 89.1% specificity.

Conclusions

These findings highlight the potential of urinary exosomal mRNA profiling, particularly focusing on RAB5B and WWP1, as a valuable strategy for improving the early detection of PCa.