Background <p>The increasing sedentary behaviour and burden of non-communicable diseases among office workers raise significant concerns. Height-adjustable sit-to-stand desks offer a potential solution, yet uptake remains challenging, limiting their effectiveness and long-term adoption. The experiences of office workers who withdrew from a height-adjustable sit-to-stand desk intervention need to be explored to inform program refinement.</p> Methods <p>A qualitative approach was used, with pre-identified themes guiding data collection, while subthemes emerged inductively from transcripts and field notes. Twelve participants with a mean age of 46.0 years were conveniently sampled. These office workers withdrew from a height-adjustable standing desk randomised controlled trial and did not form any part of other studies. One-on-one semi-structured interviews guided data collection about the participants’ experiences and reasons for early withdrawal from the intervention. Data were thematically analysed using Atlas.ti 23.</p> Results <p>Most participants were female (<i>n</i> = 10, 83.3%). All participants completed high school, while 41.7% completed a postgraduate degree from a university and credit bureau company. Despite the participants’ understanding of the benefits of height-adjustable sit-to-stand workstations, most of the participants discontinued the intervention due to perceived barriers that aligned with the themes: “discomforts and dislikes”, “applicability and practicality”, “people’s perception—i.e., what other people will say” and “transitioning to electronic sit-to-stand desks”.</p> Conclusions <p>The findings of this study may assist in formulating a policy to improve compliance with height-adjustable sit-to-stand workstations in the workplace. Future studies should consider individual preferences, design, functionality, knowledge, and motivation to ensure effective implementation, utilisation, and compliance with height-adjustable sit-to-stand workstations.</p>