Scholarlink

Objective <p>To investigate the association of peripheral blood mononuclear cell micro RNA-155 miR-155 expression with T-cell subset imbalance and disease severity in newly diagnosed paediatric immune thrombocytopenia (ITP).</p> Methods <p>Eighty-seven children with newly diagnosed ITP (43 severe, 44 non-severe) and 40 healthy controls were enrolled. Peripheral blood mononuclear cell miR-155 expression was measured via quantitative reverse transcription polymerase chain reaction; Th1, Th2, Th17 and regulatory T (Treg) cell proportions were detected using whole-blood flow cytometry and expressed as percentages of gated CD3 + CD4+ T cells. Associations and exploratory predictive performance were analysed using Pearson correlation and receiver operating characteristic curves.</p> Results <p>Peripheral blood mononuclear cell miR-155 expression was significantly higher in patients with ITP than in controls and higher in severe than in non-severe ITP (<i>P</i> &lt; 0.05); Th1 and Th17 cells, as well as Th1/Th2 and Th17/Treg ratios, were increased, whereas Th2 and Treg cells were decreased in ITP (<i>P</i> &lt; 0.05); miR-155 expression was positively associated with Th1, Th17, Th1/Th2 and Th17/Treg and negatively associated with Th2, Treg and platelet count (<i>P</i> &lt; 0.05). The exploratory combined model of miR-155, Th1/Th2 and Th17/Treg yielded an area under the curve of 0.928 (95%CI: 0.865–0.973) for severe ITP, with 79.10% sensitivity and 95.50% specificity.</p> Conclusion <p>Peripheral blood mononuclear cell miR-155 expression is associated with T-cell subset imbalance and disease severity in newly diagnosed paediatric ITP. The combined immune–cellular indicator may support severe-risk stratification, but prospective validation and functional studies are required.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Association and exploratory clinical significance of peripheral blood mononuclear cell microRNA-155 with T-Cell subset imbalance in newly diagnosed paediatric immune thrombocytopenia

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年7月24日

查看全文
  • Zhiyin Wang,
  • Xuyang Dai,
  • Peiling Li,
  • Junke Ma,
  • Yanyan Ma,
  • Youjing Tang,
  • Xueqin Tian,
  • Chen Song

摘要

Objective

To investigate the association of peripheral blood mononuclear cell micro RNA-155 miR-155 expression with T-cell subset imbalance and disease severity in newly diagnosed paediatric immune thrombocytopenia (ITP).

Methods

Eighty-seven children with newly diagnosed ITP (43 severe, 44 non-severe) and 40 healthy controls were enrolled. Peripheral blood mononuclear cell miR-155 expression was measured via quantitative reverse transcription polymerase chain reaction; Th1, Th2, Th17 and regulatory T (Treg) cell proportions were detected using whole-blood flow cytometry and expressed as percentages of gated CD3 + CD4+ T cells. Associations and exploratory predictive performance were analysed using Pearson correlation and receiver operating characteristic curves.

Results

Peripheral blood mononuclear cell miR-155 expression was significantly higher in patients with ITP than in controls and higher in severe than in non-severe ITP (P < 0.05); Th1 and Th17 cells, as well as Th1/Th2 and Th17/Treg ratios, were increased, whereas Th2 and Treg cells were decreased in ITP (P < 0.05); miR-155 expression was positively associated with Th1, Th17, Th1/Th2 and Th17/Treg and negatively associated with Th2, Treg and platelet count (P < 0.05). The exploratory combined model of miR-155, Th1/Th2 and Th17/Treg yielded an area under the curve of 0.928 (95%CI: 0.865–0.973) for severe ITP, with 79.10% sensitivity and 95.50% specificity.

Conclusion

Peripheral blood mononuclear cell miR-155 expression is associated with T-cell subset imbalance and disease severity in newly diagnosed paediatric ITP. The combined immune–cellular indicator may support severe-risk stratification, but prospective validation and functional studies are required.