Background <p>This study aimed to evaluate the information levels, satisfaction, and anxiety of parents whose infants failed the initial screening test within the National Newborn Hearing Screening Program (NNHSP) in Turkey and were subsequently referred to a referral center for further audiological assessment.</p> Methods <p>This descriptive, cross-sectional epidemiological study was conducted in Samsun Province, Turkey, and included parents of infants who failed the NNHSP screening between January 1 and December 31, 2024. Data were collected via phone interviews using a sociodemographic characteristics questionnaire, the Parent Satisfaction Questionnaire with Neonatal Hearing Screening Program (PSQ-NHSP), the State Anxiety Inventory Short Form (STAIS-5), and the Trait Anxiety Inventory Short Form (STAIT-5). The PSQ-NHSP is a 5-point Likert-type scale (scores 1 to 5). There is no established cut-off value for high satisfaction. The STAIS-5 and STAIT-5 are 4-point Likert-type scales (scores 5–20). STAIS-5 ≥ 10 and STAIT-5 ≥ 14 are considered indicative of clinical anxiety. Statistical analyses of the collected data were performed using the t-test, analysis of variance (ANOVA) test, and Pearson correlation analysis.</p> Results <p>The study included 134 parents, with a mean age of 28.37 ± 6.02 years; 82.1% were mothers. 85.5% of parents reported overall satisfaction with the NNHSP. Mean PSQ-NHSP subscale scores were 3.49 ± 0.70 for “information”, 3.63 ± 0.54 for “healthcare personnel in charge of the hearing testing”, 3.68 ± 0.50 for “appointment schedule”, and 3.84 ± 0.61 for “overall satisfaction”. PSQ-NHSP score of parents with low-income was significantly lower (<i>p</i> = 0.011) than those with high-income. PSQ-NHSP score of parents with primary-education level was significantly lower (<i>p</i> = 0.015) than those with university and above-education level. Mean STAIS-5 and STAIT-5 scores were 7.60 ± 2.16 and 8.12 ± 2.27, respectively, indicating low levels of anxiety. A significant negative correlation was found between parental satisfaction and anxiety levels (<i>p</i> &lt; 0.05).</p> Conclusions <p>Parents whose infants failed the NNHSP screening demonstrated high satisfaction but low anxiety. Ensuring adequate parental information, improving satisfaction, and addressing anxiety are essential for the effectiveness and continuity of newborn hearing screening programs.</p> Clinical trial number <p>Not applicable.</p>