Scholarlink

Background <p>Non-alcoholic fatty liver disease (NAFLD) is a growing concern among children and adolescents, with emerging evidence highlighting the role of gut microbiota in its pathogenesis. This case-control study aimed to explore the association between gut microbiota composition and NAFLD in overweight and obese Iranian children and adolescents. Given the recent updates in nomenclature, we refer to NASLD as metabolic dysfunction-associated steatotic liver disease (MASLD), in accordance with new international consensus.</p> Methods <p>A total of 66 participants, including 33 MASLD cases and 33 controls, with a case control study design were enrolled. MASLD diagnosis was done using an ultrasonography scan of the liver and gastroenterologist confirmation. Furthermore, gut microbiota composition was analyzed using real-time PCR, and metabolic, anthropometric, and dietary data were collected.</p> Results <p>The mean age of the study population (39.4% girl) was 136.82 month and mean BMI Z-score was 3.05. Regarding the gut microbiota composition, the only considerable variation was the lower abundance of Coprococcus genera in the MASLD group. Based on the analysis in the crude model and with adjustments for age, sex, and BMI, an inverse correlation was detected between the number of Coprococcus bacteria and the odds of MASLD(OR = 0.87, 95% CI: 0.77–0.98; P trend = 0.032 and OR = 0.81, 95% CI: 0.67–0.99; P trend = 0.046, respectively). However, this correlation was no longer present in the fully adjusted model. Furthermore, a negative correlation was noted between <i>Coprococcus</i> and ALT levels. On the other hand, the <i>Prevotella</i> bacteria represented significant positive correlations with both ALT and AST levels.</p> Conclusion <p>Significant differences in gut microbiota composition were observed between MASLD and control groups, including reduced SCFA-producing and increased pro-inflammatory bacteria. These findings highlight the microbiota’s potential role in MASLD pathogenesis, though confirmation in larger cohorts is needed.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

The association between gut microbiota and metabolic dysfunction-associated steatotic liver disease (MASLD): a case-control study

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年10月9日

查看全文
  • Pejman Rohani,
  • Shima Shojaie,
  • Ali Nikparast,
  • Mohsen Shaygantabar,
  • Fateme Ettehad Marvasti,
  • Golaleh Asghari,
  • Hanieh-Sadat Ejtahed,
  • Mohammad Hassan Sohouli,
  • Shirin Hasani-Ranjbar,
  • Seyed Davar Siadat,
  • Bagher Larijani,
  • Afshin Ostovar

摘要

Background

Non-alcoholic fatty liver disease (NAFLD) is a growing concern among children and adolescents, with emerging evidence highlighting the role of gut microbiota in its pathogenesis. This case-control study aimed to explore the association between gut microbiota composition and NAFLD in overweight and obese Iranian children and adolescents. Given the recent updates in nomenclature, we refer to NASLD as metabolic dysfunction-associated steatotic liver disease (MASLD), in accordance with new international consensus.

Methods

A total of 66 participants, including 33 MASLD cases and 33 controls, with a case control study design were enrolled. MASLD diagnosis was done using an ultrasonography scan of the liver and gastroenterologist confirmation. Furthermore, gut microbiota composition was analyzed using real-time PCR, and metabolic, anthropometric, and dietary data were collected.

Results

The mean age of the study population (39.4% girl) was 136.82 month and mean BMI Z-score was 3.05. Regarding the gut microbiota composition, the only considerable variation was the lower abundance of Coprococcus genera in the MASLD group. Based on the analysis in the crude model and with adjustments for age, sex, and BMI, an inverse correlation was detected between the number of Coprococcus bacteria and the odds of MASLD(OR = 0.87, 95% CI: 0.77–0.98; P trend = 0.032 and OR = 0.81, 95% CI: 0.67–0.99; P trend = 0.046, respectively). However, this correlation was no longer present in the fully adjusted model. Furthermore, a negative correlation was noted between Coprococcus and ALT levels. On the other hand, the Prevotella bacteria represented significant positive correlations with both ALT and AST levels.

Conclusion

Significant differences in gut microbiota composition were observed between MASLD and control groups, including reduced SCFA-producing and increased pro-inflammatory bacteria. These findings highlight the microbiota’s potential role in MASLD pathogenesis, though confirmation in larger cohorts is needed.