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Background <p>Diabetic retinopathy (DR), one of the most prevalent and clinically significant microvascular complications of diabetes mellitus, poses a significant challenge to public health worldwide. Circular RNAs (circRNAs) have emerged as critical regulators of the pathogenesis of various diseases.</p> Aim <p>To investigate the role and underlying molecular mechanisms of circ_0068631 in the progression of DR.</p> Methods <p>Plasma samples were collected from 80 patients with type 2 diabetes mellitus (T2DM) and 160 patients with DR, including 82 with non-proliferative DR (NPDR) and 78 with proliferative DR (PDR). circ_0068631 expression was measured by RT-qPCR, and its risk-predicitve potential for DR was evaluated using receiver operating characteristic (ROC) curves. In vitro experiments were performed using HG-treated human retinal microvascular endothelial cells (hRMECs) to assess cell function and the interaction between circ_0068631 and miR-140-5p. An enzyme-linked immunosorbent assay (ELISA) was utilized to measure the vascular endothelial growth factor (VEGF) concentration.</p> Results <p>Plasma circ_0068631 was upregulated in DR patients, especially in those with PDR, and displayed high risk-predictive value for distinguishing DR patients from T2DM (AUC = 0.913) and for identifying PDR among DR patients (AUC = 0.884). Silencing circ_0068631 inhibited viability, migration, and VEGF secretion in HG-treated hRMECs. circ_0068631 targeted miR-140-5p, and inhibiting miR-140-5p reversed the effects of circ_0068631 silencing.</p> Conclusion <p>circ_0068631 promotes DR progression by targeting miR-140-5p in hRMECs, serving as a putative blood-based marker for assessing the severity of DR and a therapeutic target for treating DR.</p>

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hsa_circ_0068631 facilitates the progression of diabetic retinopathy via regulating miR-140-5p in retinal microvascular endothelial cells

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年4月20日

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  • Bo Zhou,
  • Hua Liu,
  • Feng Xiong

摘要

Background

Diabetic retinopathy (DR), one of the most prevalent and clinically significant microvascular complications of diabetes mellitus, poses a significant challenge to public health worldwide. Circular RNAs (circRNAs) have emerged as critical regulators of the pathogenesis of various diseases.

Aim

To investigate the role and underlying molecular mechanisms of circ_0068631 in the progression of DR.

Methods

Plasma samples were collected from 80 patients with type 2 diabetes mellitus (T2DM) and 160 patients with DR, including 82 with non-proliferative DR (NPDR) and 78 with proliferative DR (PDR). circ_0068631 expression was measured by RT-qPCR, and its risk-predicitve potential for DR was evaluated using receiver operating characteristic (ROC) curves. In vitro experiments were performed using HG-treated human retinal microvascular endothelial cells (hRMECs) to assess cell function and the interaction between circ_0068631 and miR-140-5p. An enzyme-linked immunosorbent assay (ELISA) was utilized to measure the vascular endothelial growth factor (VEGF) concentration.

Results

Plasma circ_0068631 was upregulated in DR patients, especially in those with PDR, and displayed high risk-predictive value for distinguishing DR patients from T2DM (AUC = 0.913) and for identifying PDR among DR patients (AUC = 0.884). Silencing circ_0068631 inhibited viability, migration, and VEGF secretion in HG-treated hRMECs. circ_0068631 targeted miR-140-5p, and inhibiting miR-140-5p reversed the effects of circ_0068631 silencing.

Conclusion

circ_0068631 promotes DR progression by targeting miR-140-5p in hRMECs, serving as a putative blood-based marker for assessing the severity of DR and a therapeutic target for treating DR.