Purpose <p>To compare and evaluate the agreement between refractive measurements obtained by the new open-field OFA-Osiris (CSO^®) aberrometer and the subjective refraction.</p> Methods <p>Observational, prospective, cross-sectional study involving healthy eyes. Subjects underwent a subjective refraction and ocular aberrometry using the OFA-Osiris device. Sphere, cylinder, and axis values obtained from both methods were converted to polar notation using M, J0 and J45 vectors. Refractive data from ocular aberrometry were extracted at 4 and 5&#xa0;mm pupil diameters. The Wilcoxon sum-rank test was used to compare the methods, and Bland-Altman plots were used for the agreement analysis.</p> Results <p>183 eyes from 183 subjects (mean age: 24.9 ± 5.2 years) were included. No significant differences were found between the subjective and the objective refractive obtained with the OFA-Osiris aberrometer considering a pupil diameter of 5&#xa0;mm for astigmatic parameters (J0 0.01 ± 0.19 D vs. 0.02 ± 0.26 D, J45 0.03 ± 0.21 D vs. 0.01 ± 0.24, <i>p</i> &gt; 0.05) but a significant difference were observed in terms of spherical equivalent (M -1.42 ± 2.06 D vs. -1.50 ± 2.17 D, p: 0.001) When considering a pupil diameter of 4&#xa0;mm for the objective refraction measurement, no significant differences were observed in any refractive parameter (M -1.42 ± 2.06 D vs. 1.42 ± 2.25, J0 0.01 ± 0.19 D vs. 0.01 ± 0.265 D, J45 0.03 ± 0.21 D vs. 0.01 ± 0.26, <i>p</i> &gt; 0.05). Bland-Altman plots revealed 95% confidence intervals higher than 0.50 D.</p> Conclusion <p>Aberrometric refraction provided by the new open-field aberrometer, OFA-Osiris, showed general good agreement with subjective refraction – particularly for a pupil diameter of 4&#xa0;mm – but not being totally interchangeable by the presence of some unacceptable cases and recommending the subjective refraction as gold standard for refractive evaluation.</p>