Background <p>This pilot exploratory study aimed to assess the impact of a 6-month app-based multimodal visual therapy program (AmblyoPlay^®) on visual acuity (VA), stereopsis, oculomotor control, motor proficiency, and postural stability in children with anisometropic amblyopia.</p> Methods <p>This study, non-randomized controlled study included 29 children aged 7–13 years. Group I (<i>n</i> = 14) had anisometropic amblyopia and performed daily 20-minute gamified dichoptic therapy using AmblyoPlay^® for 6 months. Group II (<i>n</i> = 15) were age- and sex-matched typically developing controls without intervention. Assessments included VA (LogMAR), stereopsis (Titmus Fly), oculomotor parameters (saccadic latency, smooth pursuit gain, optokinetic gain), motor proficiency (BOT-2 subtests), and postural stability (Sensory Organization Test). Group I was evaluated at baseline, 1, 3, and 6 months; controls at baseline and 6 months. Friedman and Wilcoxon signed-rank tests were used for within-group comparisons; Mann–Whitney U tests for between-group differences.</p> Results <p>In Group I, amblyopic eye LogMAR VA improved from 0.23 ± 0.10 to 0.08 ± 0.05 at 6 months (<i>p</i> &lt; 0.001), and stereopsis improved from 494.3 ± 874.5 to 60.7 ± 28.9 arcsec (<i>p</i> &lt; 0.001). Significant reductions in saccadic latency and increases in smooth pursuit and optokinetic gains were observed (all <i>p</i> &lt; 0.05). Motor proficiency and postural stability composite scores also improved significantly (<i>p</i> &lt; 0.05). No significant changes occurred in controls.</p> Conclusions <p>AmblyoPlay-based multimodal visual therapy led to significant improvements in visual and oculomotor function, with secondary benefits in motor coordination and balance, in pediatric anisometropic amblyopia.</p> Trial registration <p>Not applicable.</p>