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Background <p>To show the representation profile and potential functions of circular RNAs (circRNAs) in a mouse model of choroidal neovascularization (CNV).</p> Methods <p>In one CNV mouse model, circRNA expression profiles were determined through microarray analysis. Selected altered circRNAs were validated by qRT-PCR. In addition to a KEGG analysis, gene ontology (GO) enrichment was implemented to measure signaling pathways, as well as biological functions. CircRNA/miRNA interactions were forecast using TargetScan and miRana programs. Choroidal flatmounts and qRT-PCR were then implemented in an attempt to examine the function of circRNA_0000273 in macrophage polarization and CNV formation.</p> Results <p>This study found that 134 circRNAs were differentially expressed within the CNV mouse model (<i>P</i>&lt; 0.05, fold change &gt; 1.5). Their host genes were found to be involved in cellular component organization (ontology: biologic reaction), organelles (ontology: cell component), and protein binding (ontology: molecular function). The mRNAs expressed with circRNAs were largely engaged in the JAK2-STAT3 signaling pathway. CircRNA_0000273 was significantly increased in M2 macrophages. Moreover, the inhibition of circRNA_0000273 was found to suppress M2 macrophage gene expression and CNV formation in the laser-induced mouse model.</p> Conclusions <p>CircRNAs are involved in CNV pathogenesis, and circRNA_0000273 is an underlying target for CNV prevention and therapy.</p>

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Identification of aberrantly expressed circular RNAs in choroidal neovascularization in mice

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年9月30日

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  • Sijie Zhao,
  • Zixuan Rao,
  • Che Xu,
  • Aiqin Wang,
  • Qing Dai,
  • Qi Zhou,
  • Jianfeng Wang

摘要

Background

To show the representation profile and potential functions of circular RNAs (circRNAs) in a mouse model of choroidal neovascularization (CNV).

Methods

In one CNV mouse model, circRNA expression profiles were determined through microarray analysis. Selected altered circRNAs were validated by qRT-PCR. In addition to a KEGG analysis, gene ontology (GO) enrichment was implemented to measure signaling pathways, as well as biological functions. CircRNA/miRNA interactions were forecast using TargetScan and miRana programs. Choroidal flatmounts and qRT-PCR were then implemented in an attempt to examine the function of circRNA_0000273 in macrophage polarization and CNV formation.

Results

This study found that 134 circRNAs were differentially expressed within the CNV mouse model (P< 0.05, fold change > 1.5). Their host genes were found to be involved in cellular component organization (ontology: biologic reaction), organelles (ontology: cell component), and protein binding (ontology: molecular function). The mRNAs expressed with circRNAs were largely engaged in the JAK2-STAT3 signaling pathway. CircRNA_0000273 was significantly increased in M2 macrophages. Moreover, the inhibition of circRNA_0000273 was found to suppress M2 macrophage gene expression and CNV formation in the laser-induced mouse model.

Conclusions

CircRNAs are involved in CNV pathogenesis, and circRNA_0000273 is an underlying target for CNV prevention and therapy.