Purpose <p>To study the added effect of sum-of-segments (SOS) biometry to modern intraocular lens power calculation formulas for eyes with short axial length.</p> Methods <p>This was a retrospective case series that included 99 eyes from 99 patients. Preoperative AXL measurements were conducted utilizing the ARGOS biometer (Alcon, Inc., Fort Worth, TX). The following formulas were used: Barrett Universal II (BUII), Cooke K6, EVO 2.0, and PEARL-DGS formulas. Additionally, the Barrett formula has been updated and is now incorporated into the ARGOS biometer, introducing the Barrett true axial length (BTAL) formula.</p> Results <p>EVO 2.0_sos and PEARL-DGS_sos formulas had the highest cases within ± 0.25 D of the intended refraction (45.45% and 42.42%, respectively). The PEARL-DGS_sos was the only formula to show a myopic mean prediction error (-0.25 ± 0.36 D). The Cooke K6 formula showed the highest hyperopic mean prediction error (0.55 ± 0.35 D), followed by EVO 2.0, Cooke K6_sos, and BUII formulas. BTAL had mean prediction error of 0.15 ± 0.47 which is less hyperopic than BUII (0.43 ± 0.39 D). Subgroup analysis of eyes with AXL 21 mm or shorter (<i>n</i> = 57) was done. Again, the PEARL-DGS_sos formula showed the only myopic mean prediction error (-0.23 ± 0.37 D).</p> Conclusion <p>PEARL-DGS_sos was the only formula to show a myopic mean prediction error. Using BTAL and SOS option in Cooke K6, EVO 2.0, and PEARL-DGS formulas decreased the undesirable hyperopic shift in the mean prediction error. This effect was more evident in shorter eyes ≤ 21.0&#xa0;mm.</p>