Scholarlink

Background <p>Cutaneous squamous cell carcinoma (cSCC) is the second most frequent cause of skin cancer death after melanoma. An improved understanding of molecular mechanisms will likely contribute to the development of improved diagnostic and treatment strategies. We investigated changes in the expression of <i>CDKN2A</i>,<i> ITGA3</i>, <i>SMAD4</i>, and <i>SIRT7</i> in human cSCC tumor tissue versus matched normal skin, and assessed their potential for use as diagnostic biomarkers.</p> Methods <p>Stage T2 (AJCC-8) cSCC tumor and normal tissue were collected (<i>n</i> = 26), qPCR was performed to detect relative target gene expression, and ROC analysis was undertaken to investigate diagnostic power of these genes. The GENT2 and DepMap were used to compare target gene expression profiles in different human cancer tissues and cell lines, and a gene-gene interaction network was generated using GeneMANIA.</p> Results <p>The qPCR indicated an increase in <i>ITGA3</i> (2.2-fold) and <i>SIRT</i>7 (1.5 fold), and a decrease in <i>CDKN2A</i> (-1.1 fold) and <i>SMAD4</i> (-0.75) expression, in cSCC versus matched normal skin. The gene-gene interaction network showed co-expression of <i>CDKN2A</i> and <i>SMAD4</i> and physical interactions between <i>ITGA3</i> and <i>SIRT7.</i> ROC analysis indicated that target gene expression could efficiently discriminate cSCC from normal skin, supporting their potential use as diagnostic molecular biomarkers.</p> Conclusions <p><i>CDKN2A</i>,<i> ITGA3</i>,<i> SMAD4</i> and <i>SIRT</i>7 genes are involved in human cSCC development and have the potential for use as diagnostic molecular biomarkers; these findings should contribute to the development of novel diagnostic and therapeutic strategies.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

CDKN2A, ITGA3, SMAD4 and SIRT7 gene expression in human cutaneous squamous cell carcinoma development and pre-clinical diagnosis

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年4月9日

查看全文
  • Karzan G. Khidhir,
  • Dana K. Sabir,
  • Abbas Salihi,
  • Benjamin H. Miranda

摘要

Background

Cutaneous squamous cell carcinoma (cSCC) is the second most frequent cause of skin cancer death after melanoma. An improved understanding of molecular mechanisms will likely contribute to the development of improved diagnostic and treatment strategies. We investigated changes in the expression of CDKN2A, ITGA3, SMAD4, and SIRT7 in human cSCC tumor tissue versus matched normal skin, and assessed their potential for use as diagnostic biomarkers.

Methods

Stage T2 (AJCC-8) cSCC tumor and normal tissue were collected (n = 26), qPCR was performed to detect relative target gene expression, and ROC analysis was undertaken to investigate diagnostic power of these genes. The GENT2 and DepMap were used to compare target gene expression profiles in different human cancer tissues and cell lines, and a gene-gene interaction network was generated using GeneMANIA.

Results

The qPCR indicated an increase in ITGA3 (2.2-fold) and SIRT7 (1.5 fold), and a decrease in CDKN2A (-1.1 fold) and SMAD4 (-0.75) expression, in cSCC versus matched normal skin. The gene-gene interaction network showed co-expression of CDKN2A and SMAD4 and physical interactions between ITGA3 and SIRT7. ROC analysis indicated that target gene expression could efficiently discriminate cSCC from normal skin, supporting their potential use as diagnostic molecular biomarkers.

Conclusions

CDKN2A, ITGA3, SMAD4 and SIRT7 genes are involved in human cSCC development and have the potential for use as diagnostic molecular biomarkers; these findings should contribute to the development of novel diagnostic and therapeutic strategies.