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Objective <p>RNA-seq was used to explore the potential mechanism underlying human leukocyte-associated immunoglobulin-like receptor 1 (LAIR-1) inhibition of the proliferation and migration of ovarian cancer cells.</p> Method <p>A LAIR-1–overexpression cell model was established using a LAIR-1-lentivirus. After confirming and identifying the LAIR-1 expression cell clones by flow cytometry and RT-qPCR, the proliferation and migration of the cells were examined by CCK8 and scratch assays, and the differentially expressed genes (DEGs) were searched by RNA-seq and analyzed by GO and KEGG enrichment. String was used for protein interaction network analysis, and Cytoscape was used to identify key proteins.</p> Results <p>LAIR-1 inhibited the proliferation and migration of ovarian cancer cells. LAIR-1 expression caused the upregulation of 83 genes and the downregulation of 80 genes. Among the DEGs, fibronectin 1 (FN1) was a key protein affecting the downstream FAK-MEK-ERK axis. KEGG enrichment analysis identified the MAPK pathway as the most obvious enrichment pathway, followed by PI3K-AKT pathway.</p> Conclusion <p>LAIR-1 downregulates FN1 to inhibit the FAK-MEK-ERK axis, as well as the proliferation and migration of ovarian cancer cells.</p>

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Mechanistic role of FN1 in LAIR-1 mediated downregulation of ovarian cancer cell proliferation

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年2月25日

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  • Huachang Zhang,
  • Mengke Liu,
  • Hao Zhong,
  • Li Ma,
  • Yunyi Liu,
  • Chuntong Liu,
  • Shude Yang,
  • Bin Wang,
  • Jing An,
  • Shuling Song,
  • Qizhi Cao

摘要

Objective

RNA-seq was used to explore the potential mechanism underlying human leukocyte-associated immunoglobulin-like receptor 1 (LAIR-1) inhibition of the proliferation and migration of ovarian cancer cells.

Method

A LAIR-1–overexpression cell model was established using a LAIR-1-lentivirus. After confirming and identifying the LAIR-1 expression cell clones by flow cytometry and RT-qPCR, the proliferation and migration of the cells were examined by CCK8 and scratch assays, and the differentially expressed genes (DEGs) were searched by RNA-seq and analyzed by GO and KEGG enrichment. String was used for protein interaction network analysis, and Cytoscape was used to identify key proteins.

Results

LAIR-1 inhibited the proliferation and migration of ovarian cancer cells. LAIR-1 expression caused the upregulation of 83 genes and the downregulation of 80 genes. Among the DEGs, fibronectin 1 (FN1) was a key protein affecting the downstream FAK-MEK-ERK axis. KEGG enrichment analysis identified the MAPK pathway as the most obvious enrichment pathway, followed by PI3K-AKT pathway.

Conclusion

LAIR-1 downregulates FN1 to inhibit the FAK-MEK-ERK axis, as well as the proliferation and migration of ovarian cancer cells.