Scholarlink

Background <p>To understand the genetic diversity and recombination of norovirus infection in children with hospital-acquired acute gastroenteritis in Shanghai, and to provide a scientific basis for the prevention of norovirus infection in hospitalized children.</p> Methods <p>12,485 stool specimens of children with hospital-acquired acute gastroenteritis were collected from October 2018 to June 2021. Gene amplification and sequencing of VP1, RdRp and RdRp-VP1 region were performed on norovirus-positive specimens using RT-PCR method. Genotyping and recombination analysis of sequenced sequences were performed using online analysis tools.</p> Results <p>The positive rate of norovirus infection in children with hospital-acquired diarrhea by real-time quantitative RT-PCR was 16.2% (2,017/12,485). Based on the gene sequencing analysis of norovirus across the RdRp-VP1 region, nine norovirus recombinant strains were detected. From October 2018 to September 2020, the proportion of norovirus GII.4 Sydney_2012[P31] was 56.5% (231/409), while the proportion of GII.4 Sydney_2012[P16] was only 1.2% (5/409) in the same period. However, from October 2020 to June 2021, the detection rate of the recombinant strain GII.4 Sydney_2012[P16] increased to 38.8% (57/147), which exceeded that of the recombinant strain GII.4 Sydney_2012[P31] (19.0%, 28/147).</p> Conclusions <p>Norovirus GII.4 Sydney_2012 remained the main prevalent genotype, but the recombinant strain of GII.4 Sydney_2012[P16] had an increased positivity rate since October 2020. It is imperative that surveillance and management of hospital-acquired norovirus acute gastroenteritis in hospitalized children be strengthened to prevent nosocomial norovirus outbreaks.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

The increased detection of recombinant norovirus GII.4 Sydney_2012[P16] strains in hospitalized pediatric patients with gastroenteritis in Shanghai, China

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年1月28日

查看全文
  • Fangyuan Yu,
  • Xuhua Feng,
  • Haiqin Ye,
  • Qin Cai,
  • Jie Jiang,
  • Tiandong Zhang,
  • Fen Pan,
  • Wenhao Weng

摘要

Background

To understand the genetic diversity and recombination of norovirus infection in children with hospital-acquired acute gastroenteritis in Shanghai, and to provide a scientific basis for the prevention of norovirus infection in hospitalized children.

Methods

12,485 stool specimens of children with hospital-acquired acute gastroenteritis were collected from October 2018 to June 2021. Gene amplification and sequencing of VP1, RdRp and RdRp-VP1 region were performed on norovirus-positive specimens using RT-PCR method. Genotyping and recombination analysis of sequenced sequences were performed using online analysis tools.

Results

The positive rate of norovirus infection in children with hospital-acquired diarrhea by real-time quantitative RT-PCR was 16.2% (2,017/12,485). Based on the gene sequencing analysis of norovirus across the RdRp-VP1 region, nine norovirus recombinant strains were detected. From October 2018 to September 2020, the proportion of norovirus GII.4 Sydney_2012[P31] was 56.5% (231/409), while the proportion of GII.4 Sydney_2012[P16] was only 1.2% (5/409) in the same period. However, from October 2020 to June 2021, the detection rate of the recombinant strain GII.4 Sydney_2012[P16] increased to 38.8% (57/147), which exceeded that of the recombinant strain GII.4 Sydney_2012[P31] (19.0%, 28/147).

Conclusions

Norovirus GII.4 Sydney_2012 remained the main prevalent genotype, but the recombinant strain of GII.4 Sydney_2012[P16] had an increased positivity rate since October 2020. It is imperative that surveillance and management of hospital-acquired norovirus acute gastroenteritis in hospitalized children be strengthened to prevent nosocomial norovirus outbreaks.