Scholarlink

Background <p>Hospital-acquired infections caused by multidrug resistant (MDR) <i>Klebsiella pneumoniae</i> pose a significant global health threat. Effective antisepsis and disinfection protocols are mandatory to prevent these infections. This study aimed to isolate <i>Klebsiella pneumoniae</i>, evaluate antimicrobial susceptibility, and assess the efficacy of selected biocides.</p> Methods <p>Fifty clinical MDR <i>Klebsiella pneumoniae</i> isolates were collected from various hospital departments. Antimicrobial susceptibility was determined using the disc diffusion method. Minimum inhibitory concentrations (MICs) of chlorhexidine and benzalkonium chloride were measured via agar dilution. Conventional PCR was employed to detect biocide resistance genes (<i>qacE∆1</i> and <i>cepA</i>).</p> Results <p><i>Klebsiella pneumoniae</i> was identified in 19.16% of cases. All isolates exhibited multidrug resistance, with multiple antimicrobial resistance indices ranging from 0.24 to 0.92, reaching up to 1. Benzalkonium chloride MICs significantly increased with resistance, reaching up to 64&#xa0;µg/mL, while chlorhexidine MICs were consistent across isolates. The <i>qacE∆1</i> and <i>cepA</i> genes were detected in 62% and 72% of isolates, respectively, with a significant association between <i>qacE∆1</i> and cephalosporin resistance. No significant correlation was found between biocide MICs and clinical specimen types or hospital units.</p> Conclusion <p>The <i>cepA</i> gene is closely associated with extensive drug resistance in <i>Klebsiella pneumoniae</i>, emphasizing its role in antimicrobial resistance. Optimized biocide formulations, when properly developed and applied, can play a crucial role in combating and preventing infections caused by multidrug-resistant <i>Klebsiella pneumoniae</i>.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Chlorhexidine and benzalkonium chloride: promising adjuncts in combating multidrug resistant Klebsiella pneumoniae in healthcare settings

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年5月7日

查看全文
  • Amal F. Makled,
  • Azza Z. Labeeb,
  • Heba M. Moaz,
  • Asmaa S. Sleem

摘要

Background

Hospital-acquired infections caused by multidrug resistant (MDR) Klebsiella pneumoniae pose a significant global health threat. Effective antisepsis and disinfection protocols are mandatory to prevent these infections. This study aimed to isolate Klebsiella pneumoniae, evaluate antimicrobial susceptibility, and assess the efficacy of selected biocides.

Methods

Fifty clinical MDR Klebsiella pneumoniae isolates were collected from various hospital departments. Antimicrobial susceptibility was determined using the disc diffusion method. Minimum inhibitory concentrations (MICs) of chlorhexidine and benzalkonium chloride were measured via agar dilution. Conventional PCR was employed to detect biocide resistance genes (qacE∆1 and cepA).

Results

Klebsiella pneumoniae was identified in 19.16% of cases. All isolates exhibited multidrug resistance, with multiple antimicrobial resistance indices ranging from 0.24 to 0.92, reaching up to 1. Benzalkonium chloride MICs significantly increased with resistance, reaching up to 64 µg/mL, while chlorhexidine MICs were consistent across isolates. The qacE∆1 and cepA genes were detected in 62% and 72% of isolates, respectively, with a significant association between qacE∆1 and cephalosporin resistance. No significant correlation was found between biocide MICs and clinical specimen types or hospital units.

Conclusion

The cepA gene is closely associated with extensive drug resistance in Klebsiella pneumoniae, emphasizing its role in antimicrobial resistance. Optimized biocide formulations, when properly developed and applied, can play a crucial role in combating and preventing infections caused by multidrug-resistant Klebsiella pneumoniae.