Remote surveillance and detection of SARS-CoV-2 transmission among household members in King County, Washington

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2024年3月13日

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Anne Emanuels,
Amanda M. Casto,
Jessica Heimonen,
Jessica O’Hanlon,
Eric J. Chow,
Constance Ogokeh,
Melissa A. Rolfes,
Peter D. Han,
James P. Hughes,
Timothy M. Uyeki,
Christian Frazar,
Erin Chung,
Lea M. Starita,
Janet A. Englund,
Helen Y. Chu,
Michael Boeckh,
Michael Famulare,
Barry R. Lutz,
Deborah A. Nickerson,
Mark J. Rieder,
Matthew Thompson,
Jay Shendure,
Trevor Bedford,
Amanda Adler,
Elisabeth Brandstetter,
Roy Burstein,
Shari Cho,
Rachel E. Geyer,
James Hadfield,
Michael L. Jackson,
Anahita Kiavand,
Ashley E. Kim,
Louise E. Kimball,
Jack Henry Kotnik,
Kirsten Lacombe,
Jennifer K. Logue,
Victoria Lyon,
Denise J. McCulloch,
Matthew Richardson,
Julia H. Rogers,
Thomas R. Sibley,
Monica L. Zigman Suchsland,
Melissa Truong,
Caitlin R. Wolf,
Weizhi Zhong

摘要

Background

Early during the COVID-19 pandemic, it was important to better understand transmission dynamics of SARS-CoV-2, the virus that causes COVID-19. Household contacts of infected individuals are particularly at risk for infection, but delays in contact tracing, delays in testing contacts, and isolation and quarantine posed challenges to accurately capturing secondary household cases.

Methods

In this study, 346 households in the Seattle region were provided with respiratory specimen collection kits and remotely monitored using web-based surveys for respiratory illness symptoms weekly between October 1, 2020, and June 20, 2021. Symptomatic participants collected respiratory specimens at symptom onset and mailed specimens to the central laboratory in Seattle. Specimens were tested for SARS-CoV-2 using RT-PCR with whole genome sequencing attempted when positive. SARS-CoV-2-infected individuals were notified, and their household contacts submitted specimens every 2 days for 14 days.

Results

In total, 1371 participants collected 2029 specimens that were tested; 16 individuals (1.2%) within 6 households tested positive for SARS-CoV-2 during the study period. Full genome sequences were generated from 11 individuals within 4 households. Very little genetic variation was found among SARS-CoV-2 viruses sequenced from different individuals in the same household, supporting transmission within the household.

Conclusions

This study indicates web-based surveillance of respiratory symptoms, combined with rapid and longitudinal specimen collection and remote contact tracing, provides a viable strategy to monitor households and detect household transmission of SARS-CoV-2.

Trial registration identifier

NCT04141930, Date of registration 28/10/2019.